Phenotype Image Detail

Image

Caption

Unusual biochemical properties and morphology of Pla2g3^tm1Murm/Pla2g3^tm1Murm sperm. (A) ATP contents of capacitated sperm from wild-type (+/+), Pla2g3^tm1Murm/Pla2g3⁺ (+/-), and Pla2g3^tm1Murm/Pla2g3^tm1Murm (-/-) mice (mean +/- SD; n = 8; *P < 0.05). (B and C) Sperm from wild-type and homozygous mutant mice were incubated for the indicated periods in HTF medium, and 10⁶ sperm cell equivalents were subjected to SDS-PAGE/immunoblotting with antiphosphotyrosine antibody. (B) Arrows on the right margin indicate the bands that were increased during capacitation and reduced in homozygous mutant mice compared with wild-type mice, and arrows on the left indicate molecular weight. Blot lanes were run on the same gels but were noncontiguous. (C) Inducible phosphorylation of 80-kDa protein relative to constitutive phosphorylation of 115-kDa hexokinase was calculated, with the intensity at 90 minutes being regarded as 100% (mean +/- SD; n = 5; *P < 0.05, **P < 0.01). (D) Homogenates of testis and epididymis (epi) of wild-type and homozygous mutant mice (20 ug protein) were subjected to SDS-PAGE/immunoblotting with anti-fertilin beta antibody. Arrows indicate 100-, 45-, and 30-kDa forms. (E) Morphology of sperm from wild-type and homozygous mutant mice under light microscopy (original magnification, x200). Homozygous mutant sperm frequently showed a round-shaped head (arrowheads) as well as coiled (filled arrow), bent (dashed arrow), and duplicate (open arrow) tails. Sperm heads are magnified in the insets. Scale bar: 10 um. (F) The proportions of cauda epididymal spermatozoa with or without defects in head morphology were evaluated (mean +/- SD; n = 7; *P < 0.05, **P < 0.01).

Copyright

This image is from Sato H, J Clin Invest 2010 May 3;120(5):1400-14 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161462

Associated Alleles

Symbol	Name
Pla2g3^tm1Murm	phospholipase A2, group III; targeted mutation 1, Makoto Murakami

Associated Genotypes

Allelic Composition	Genetic Background
Pla2g3^tm1Murm/Pla2g3^tm1Murm	involves: 129S/SvEv * C57BL/6J