Phenotype Image Detail

Image

Caption

(A) Representative view showing splenomegaly in Sos1^tm1.2Rak/\AlleleSymbol (MGI:2176916|0) (+/EK) mice, with quantification graphed (n = 8). Error bars indicate SD. **P < 0.01. (B) Representative Hematoxylin & Eosin (H&E) stained sections of spleens, showing increased numbers of megakaryocytes (white arrowheads) and massive increase of red pulp (bottom panel) in the mutant mice. Immunohistochemistry staining showing CD41-positive megakaryocytes (brown cells; right panels). Original magnification, x75. (C) Blood smears showing an increased number of neutrophils in mutant mice. Original magnification, x100. (D) Flow cytometric analysis of bone marrow and spleen, showing increased percentages of myeloid lineage (Mac1+ and Mac1+Gr1+) cells in mutant mice (P < 0.05 for bone marrow; P < 0.01 for spleen). Numbers indicate the percentage of positively-stained cells. (E) Increased myeloid colony formation of mutant bone marrow in the absence or presence of the indicated concentrations of GM-CSF or IL-3 (P < 0.05 and P < 0.01, respectively).

Copyright

This image is from Chen PC, J Clin Invest 2010 Dec 1;120(12):4353-65 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:171873

Associated Alleles

Symbol	Name
Sos1^tm1.2Rak	SOS Ras/Rac guanine nucleotide exchange factor 1; targeted mutation 1.2, Raju Kucherlapati

Associated Genotypes

Allelic Composition	Genetic Background
Sos1^tm1.2Rak/Sos1^+	involves: 129S/Sv * C57BL/6 * FVB/N * SJL