Phenotype Image Detail

Image

Caption

Ultrastructure of the node of Ranvier, paranode, and Schmidt-Lanterman incisures in Sbf2^{Gt(RRF511)Byg}/Sbf2^{Gt(RRF511)Byg} (KO) nerve fibers. Wild-type (WT; F,I, and M) and mutant (A-E, G,H and J-L) mice were analyzed at 2 (A-C) or 7 (D-M) months. Longitudinal sections (0.5 um and 70 nm in A-C and D-M, respectively) of sciatic nerves were analyzed by light microscopy (A-C) or EM (D-M). (A-C) Myelin misfolding is most prominent near nodes of Ranvier (A) but also occurs in internodal regions (arrows in B and C). (D) Myelin outfoldings adjacent to a node of Ranvier (NR) in an Sbf2^{Gt(RRF511)Byg}/Sbf2^{Gt(RRF511)Byg} nerve fiber. Note that the Schwann cell on either side of the node is affected. (E and F) Nodal microvilli (mv) appear morphologically normal in mutant Schwann cells. E is a magnification of the indicated portion of D. The electron-dense undercoating of the nodal axolemma (arrowhead) appears morphologically normal in mutant Schwann cells. The overlaying basal lamina is intact in nodes of Ranvier in mutant nerve fibers (a dotted line traces the outer edge of the basal lamina in E). (G-I) Paranodal, septate-like axo-glial junctions (arrowheads) are intact in mutant nerve fibers. H is a magnification of the indicated portion of G. (I and J) Autotypic adherens junctions (arrows) within paranodal loops are intact in mutant Schwann cells. (K-M) Autotypic adherens junctions (arrows) within Schmidt-Lanterman incisures are intact and in register in mutant Schwann cells. L is a magnification of the indicated portion of K. [Scale bar: 29 um (A-C); 3.9 um (D), 523 nm (E and F), 2 um (G), 200 nm (H), 327 nm (I), 391 nm (J), 5.2 um (K), 169 nm (L), and 200 nm (M).]

Copyright

This image is from Robinson FL, Proc Natl Acad Sci U S A 2008 Mar 25;105(12):4916-21. Copyright 2008 National Academy of Sciences, U.S.A. J:133391

Associated Alleles

Symbol	Name
Sbf2^Gt(RRF511)Byg	SET binding factor 2; gene trap RRF511, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Sbf2^Gt(RRF511)Byg/Sbf2^Gt(RRF511)Byg	involves: 129P2/OlaHsd * C57BL/6