Phenotype Image Detail

Image

Caption

Transmission electron micrographs of FDL (flexor digitorum longus) tendon at P4 from (a) wild-type (+/+) and (b) Col14a1^tm1Debi/Col14a1^tm1Debi (-/-) mice. At P4, the fibrils of the wild-type control are more uniform in shape and smaller than the fibrils in the mutant tendon, which are more irregular and larger. At P60, the mature fibrils from the wild-type tendon are of variable size with small to larger cross sectional profiles. In the mutant tendon, there are fewer smaller fibrils and more fibrils of larger diameter as compared with the wild-type control. Bar, 100 nm. C, histograms representing fibril diameter measurements of tendons at P4 and P60. In the P4 wild-type tendon, the fibril diameter distribution demonstrates a unimodal pattern with the majority of fibril diameters between 35 and 45 nm. In the P4 mutant tendon, the fibril diameter measurements also show a unimodal (50-60 nm) distribution; however, there is a greater frequency of larger diameter fibrils. At P60, the wild-type diameter measurements have a much broader multimodal distribution. The fibril diameter measurements in the mutant tendon also have a multimodal distribution. In contrast to the wild-type distribution, there is a reduced frequency of smaller diameter fibrils in the mutant tendons. In addition, a distinct subpopulation of larger diameter fibrils, absent in the wild-type tendons, is present in the mutant tendons. Suggesting this represents the fibrils that prematurely enter the fibril growth phase in the absence of type XIV collagen.

Copyright

This image is from Ansorge HL, J Biol Chem 2009 Mar 27;284(13):8427-38 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:148619

Associated Alleles

Symbol	Name
Col14a1^tm1Debi	collagen, type XIV, alpha 1; targeted mutation 1, David E Birk

Associated Genotypes

Allelic Composition	Genetic Background
Col14a1^tm1Debi/Col14a1^tm1Debi	involves: 129S4/SvJae * C57BL/6