Phenotype Image Detail

Image

Caption

A,B: H&E stained sections of eyes from E12.5 wild-type and Pds5a^{Gt(RRM243)Byg}/Pds5a^{Gt(RRM243)Byg} Pds5b^tm1Jmi/Pds5b⁺ embryos. No lens was formed in the Pds5a^{Gt(RRM243)Byg}/Pds5a^{Gt(RRM243)Byg} Pds5b^tm1Jmi/Pds5b⁺ embryo (B), whereas the wild-type embryo has a clearly formed lens at this stage (A). C,D: Whole mount image of the eyes from E11.5 wild-type and mutant embryos. Note that the Pds5a^{Gt(RRM243)Byg}/Pds5a⁺ Pds5b^tm1Jmi/Pds5b^tm1Jmi eye (arrow, D) is much smaller than wild-type (white dashed circle, C). E: Immunofluorescent staining of E9.5 eye using PDS5A antibodies demonstrated that PDS5A is expressed at high levels in head surface ectoderm. HSE, head surface ectoderm; M, mesenchyme; OV, optical vesicle; dashed lines, the boundaries among HSE, M, and OV. F-H: Proliferating cells in the E10.5 eyes of wild-type (G) and Pds5a^{Gt(RRM243)Byg}/Pds5a^{Gt(RRM243)Byg} Pds5b^tm1Jmi/Pds5b⁺ (H) embryos labeled with BrdU for 1 hr were detected using anti-BrdU antibodies. The eyes were counterstained with hematoxylin. Eye structures are as indicated in G. Note that the lens placode is invaginating in wild-type (G) but not in Pds5a^{Gt(RRM243)Byg}/Pds5a^{Gt(RRM243)Byg} Pds5b^tm1Jmi/Pds5b⁺ embryos (H), although the percentage of BrdU positive cells in both wild-type and mutant lens placodes was equivalent (F). I,J: Immunohistochemical analysis using alphaA-crystallin anitbodies of E11.5 wild-type and Pds5a^{Gt(RRM243)Byg}/Pds5a⁺ Pds5b^tm1Jmi/Pds5b^tm1Jmi eyes. Note the small size and lack of alphaA-crystallin-positive cells in a Pds5a^{Gt(RRM243)Byg}/Pds5a⁺ Pds5b^tm1Jmi/Pds5b^tm1Jmi lens (J) compared to a strong alphaA-crystallin expression in the majority of cells in a normal size wild-type lens (I). R, retina; L, lens.

Copyright

This image is from Zhang B, PLoS ONE 2009;4(5):e5232, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148890

Associated Alleles

Symbol	Name
Pds5a^Gt(RRM243)Byg	PDS5 cohesin associated factor A; gene trap RRM243, BayGenomics
Pds5b^tm1Jmi	PDS5 cohesin associated factor B; targeted mutation 1, Jeffrey Milbrandt

Associated Genotypes

Allelic Composition	Genetic Background
Pds5a^Gt(RRM243)Byg/Pds5a^+ Pds5b^tm1Jmi/Pds5b^tm1Jmi	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
Pds5a^Gt(RRM243)Byg/Pds5a^Gt(RRM243)Byg Pds5b^tm1Jmi/Pds5b^+	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6