Phenotype Image Detail

Image

Caption

Kidney development. Paraffin-embedded tissue sections were generated from fixed tissue isolated from wild type (+/+) and Gpc3^tm1Fil/Y (-/) mice. E12.0 sections were imaged at 200x. Bar, 37.5um. E13.5, E16.5, and E18.5 sections were imaged at 100x. Bar, 75um. At E12.0, branching of the ureteric bud is markedly enhanced in the hemizygous versus wild type embryo (arrowheads). By E13.5, the mutant kidney is much larger than that of its wild type littermate and consists of histologically normal ureteric bud and mesenchymal-derived tissue elements. While these mesenchymal-derived elements (glomeruli and tubules) are present in the cortex (C) of the E16.5 mutant and wild type kindeys, they are disorganized in the mutant cortex and the medulla (M) is relatively devoid of tubular structures. Cysts (Cy) are present in the mutant medulla in an irregular pattern. At E18.5, these abnormalities persist in the mutant kidney and are accompanied by total absence of the medullary tubular patterning characteristic of the wild type kidney.

Copyright

This image is from Cano-Gauci DF, J Cell Biol 1999 Jul 12;146(1):255-64, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:73877

Associated Alleles

Symbol	Name
Gpc3^tm1Fil	glypican 3; targeted mutation 1, Jorge Filmus

Associated Genotypes

Allelic Composition	Genetic Background
Gpc3^tm1Fil/Y	B6.Cg-Gpc3^tm1Fil