Phenotype Image Detail

Image

Caption

A-D: Hematoxylin and eosin staining of livers from wild type (A and C) and Fstl3^tm1Alsc/Fstl3^tm1Alsc (B and D) mice. E and F: Frozen sections of liver stained with Oil Red O from wild type (E) and mutant (F) mice at 9.5 months. G-H: Liver sections stained with Periodic acid-Schiff's stain showing vastly reduced glycogen content in mutant mice (H) compared with wild type littermates (G). Magnifications: A and B, x10; C-H, x40, Scale bars, A and B; 100um; C-H, 50um. I: Gluconeogenic genes PEPCK and G6Pase are significantly (P<0.05) elevated in muant mice relative to wild type mice by 6-and 4.5-fold, respectively, as analyzed by quantitative PCR. J: Activin (5 ng/ml) significantly (P<0.01) stimulated G6Pase gene expression relative to untreated HepG2 liver hepatoma cells, whereas myostain (25 ng/ml) supressed G6Pase under the same conditions (P<0.05).

Copyright

This image is from Mukherjee A, Proc Natl Acad Sci U S A 2007 Jan 23;104(4):1348-53. Copyright 2007 National Academy of Sciences, U.S.A. J:119526

Associated Alleles

Symbol	Name
Fstl3^tm1Alsc	follistatin-like 3; targeted mutation 1, Alan Schneyer

Associated Genotypes

Allelic Composition	Genetic Background
Fstl3^tm1Alsc/Fstl3^tm1Alsc	involves: 129S4/SvJae * C57BL/6 * FVB/N