Phenotype Image Detail

Image

Caption

Toluidine blue staining of sagittal sections of distal femurs. (A-E) Control femurs at 1 week (A) and 3 weeks (D) of age. Boxed areas in (A) are enlarged in (B) and (C). Boxed area in (D) is enlarged in (E). Blue arrows in (C) point to osteoblast cells lining the surface of cortical bone. Pink arrows in (G) show similar cells to those in (C) in Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 femurs. Red star in (A) and (D) marks the secondary ossification center. Pale bluestained tissue in (E), marked by red arrows, is trabecular bone. bm, marks the bone marrow cavity in (D). (F-J) Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 at 1 week (F) and 3 weeks (J) of age. Boxed areas in (F) are enlarged in (G), (H), and (I). (A), (D), (F), and (J) are shown with the same magnification. Note that there are defects in bone formation but no defect in osteoclast mediated bone resorption. Mineralized tissue in the mid shaft of femur is almost resorbed at 3 wks. (H) Fibroblast-like cells present in the bone shaft of Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 mouse. Red star in (I) shows that osteoclasts invading the Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 femurs at 1 week of age. s and m mark soft tissues and muscles in (J), respectively. (K & L) Three week old wild type (K) and Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 (L) hindlimb skeletons stained with Alcian blue and Alizarin red. The black arrow shows the missing part of proximal femur.

Copyright

This image is from Bandyopadhyay A, PLoS Genet 2006 Dec 22;2(12):e216, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:118257

Associated Alleles

Symbol	Name
Bmp2^tm1Cjt	bone morphogenetic protein 2; targeted mutation 1, Clifford J Tabin
Bmp4^tm1Jfm	bone morphogenetic protein 4; targeted mutation 1, James F Martin
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin

Associated Genotypes

Allelic Composition	Genetic Background
Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0	involves: 129S4/SvJaeSor * C57BL/6 * SJL