Phenotype Image Detail

Image

Caption

(A) Hematoxylin and eosin stain showing red blood cells (arrowheads) outside a blood vessel in the neck region at E13.5. (B) A skin capillary at E13.5 from a region not showing gross hemorrhage. A red blood cell (arrowhead and Inset) is seen traversing the endothelial cell lining between two adjacent cells. (C-F) Tissue sections revealing the leak of intravascularly injected fluorescent dextran into the extravascular interstitium (D, arrows) at distant sites in a Pkd1^tm1Maa/Pkd1^tm1Maa embryo (D & F). In the age matched wild type (C and E), dextran is retained within the vasculature (arrows). No fluorescence is seen on the basolateral side of the lining endothelium in wild type embryos (E, arrows). In contrast, fluorescent dextran is seen basolaterally (F, arrows) and at intercellular junctions in the mutant. (G-I) Hematoxylin and eosin stain of a section of a normal developing kidney at E14.5 (G), showing two normal glomeruli (arrowheads). An age-matched mutant kidney (H) shows two large glomerular cysts (arrows), and a tubular cyst (arrowhead). (I) A section of a pancreas from the mutant embryo shows two tubular cysts (arrows) adjacent to normal-sized ducts (arrowhead). Sections shown in A, B, G, H, and I are 5 m thick, and those in C-F are 4 m thick. (Scale bars = 10 m, except in E and F where the scale bar = 5 m.)

Copyright

This image is from Kim K, Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1731-6. Copyright 2000 National Academy of Sciences, U.S.A. J:68331

Associated Alleles

Symbol	Name
Pkd1^tm1Maa	polycystin 1, transient receptor potential channel interacting; targeted mutation 1, M Armin Arnout

Associated Genotypes

Allelic Composition	Genetic Background
Pkd1^tm1Maa/Pkd1^tm1Maa	involves: 129S4/SvJae * C57BL/6