Gene Expression Literature Summary

• Symbol: Afdn
• Name: afadin, adherens junction formation factor
• ID: MGI:1314653

43 matching records from 43 references.

Summary by Age and Assay: Numbers in the table indicate the number of results matching the search criteria.

Age	E6.5	E7	E7.5	E8	E8.5	E9	E9.5	E10.5	E11	E11.5	E12.5	E13.5	E14.5	E15.5	E16.5	E17.5	E18.5	E	P
Immunohistochemistry (section)	2		3	1	1	1	2	3	1	5	5	11	7	5	4	1	5	2	5
In situ RNA (section)										1	1	1	1
Immunohistochemistry (whole mount)	1	1	1		2		2						1		1				2
In situ RNA (whole mount)			1				2	1		1			1	2
Western blot					1			1			2	3	3	2	1	1	2		3
RT-PCR															1		1		1
cDNA clones							1			1

Summary by Gene and Reference: Number indicates the number of results matching the search criteria recorded for each reference.
* Indicates detailed expression data entries available

Afdn  afadin, adherens junction formation factor   (Synonyms: 5033403D15Rik, AF6, Afadin, I-afadin, Mllt4, S-afadin)
Results	Reference
1	J:330604 Akturk A, Day M, Tarchini B, RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells. Sci Adv. 2022 Oct 21;8(42):eabq2826
5	J:260459 Azizoglu DB, Braitsch C, Marciano DK, Cleaver O, Afadin and RhoA control pancreatic endocrine mass via lumen morphogenesis. Genes Dev. 2017 Dec 1;31(23-24):2376-2390
1	J:226997 Bebee TW, Park JW, Sheridan KI, Warzecha CC, Cieply BW, Rohacek AM, Xing Y, Carstens RP, The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development. Elife. 2015;4
1*	J:152211 Coulpier F, Le Crom S, Maro GS, Manent J, Giovannini M, Maciorowski Z, Fischer A, Gessler M, Charnay P, Topilko P, Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers. Glia. 2009 Oct;57(13):1450-7
3	J:171148 Daane JM, Enders AC, Downs KM, Mesothelium of the murine allantois exhibits distinct regional properties. J Morphol. 2011 May;272(5):536-56
3	J:271772 Dewitz C, Pimpinella S, Hackel P, Akalin A, Jessell TM, Zampieri N, Nuclear Organization in the Spinal Cord Depends on Motor Neuron Lamination Orchestrated by Catenin and Afadin Function. Cell Rep. 2018 Feb 13;22(7):1681-1694
1*	J:153498 Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nurnberger A, SchmidtK, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
2	J:206576 Fukuda T, Kominami K, Wang S, Togashi H, Hirata K, Mizoguchi A, Rikitake Y, Takai Y, Aberrant cochlear hair cell attachments caused by Nectin-3 deficiency result in hair bundle abnormalities. Development. 2014 Jan;141(2):399-409
1*	J:245763 Gao L, Yang Z, Hiremath C, Zimmerman SE, Long B, Brakeman PR, Mostov KE, Bryant DM, Luby-Phelps K, Marciano DK, Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
2	J:201651 Gil-Sanz C, Franco SJ, Martinez-Garay I, Espinosa A, Harkins-Perry S, Muller U, Cajal-Retzius cells instruct neuronal migration by coincidence signaling between secreted and contact-dependent guidance cues. Neuron. 2013 Aug 7;79(3):461-77
1	J:215584 Gil-Sanz C, Landeira B, Ramos C, Costa MR, Muller U, Proliferative defects and formation of a double cortex in mice lacking Mltt4 and Cdh2 in the dorsal telencephalon. J Neurosci. 2014 Aug 6;34(32):10475-87
1*	J:91257 Gray PA, Fu H, Luo P, Zhao Q, Yu J, Ferrari A, Tenzen T, Yuk DI, Tsung EF, Cai Z, Alberta JA, Cheng LP, Liu Y, Stenman JM, Valerius MT, Billings N, Kim HA, Greenberg ME, McMahon AP, Rowitch DH, Stiles CD, Ma Q, Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis. Science. 2004 Dec 24;306(5705):2255-2257
1*	J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
1*	J:337132 Hiremath C, Gao L, Geshow K, Patterson Q, Barlow H, Cleaver O, Marciano DK, Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
4	J:57589 Ikeda W, Nakanishi H, Miyoshi J, Mandai K, Ishizaki H, Tanaka M, Togawa A, Takahashi K, Nishioka H, Yoshida H, Mizoguchi A, Nishikawa S, Takai Y, Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis. J Cell Biol. 1999 Sep 6;146(5):1117-32
3	J:101707 Inagaki M, Irie K, Ishizaki H, Tanaka-Okamoto M, Morimoto K, Inoue E, Ohtsuka T, Miyoshi J, Takai Y, Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development. 2005 Apr;132(7):1525-37
4*	J:61349 Kanai-Azuma M, Mattick JS, Kaibuchi K, Wood SA, Co-localization of FAM and AF-6, the mammalian homologues of Drosophila faf and canoe, in mouse eye development. Mech Dev. 2000 Mar 1;91(1-2):383-6
1	J:222380 Katsuno T, Umeda K, Matsui T, Hata M, Tamura A, Itoh M, Takeuchi K, Fujimori T, Nabeshima Y, Noda T, Tsukita S, Tsukita S, Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. Mol Biol Cell. 2008 Jun;19(6):2465-75
2	J:102364 Koike C, Nishida A, Akimoto K, Nakaya MA, Noda T, Ohno S, Furukawa T, Function of atypical protein kinase Clambda in differentiating photoreceptors is required for proper lamination of mouse retina. J Neurosci. 2005 Nov 2;25(44):10290-8
2*	J:180113 Lachke SA, Higgins AW, Inagaki M, Saadi I, Xi Q, Long M, Quade BJ, Talkowski ME, Gusella JF, Fujimoto A, Robinson ML, Yang Y, Duong QT, Shapira I, Motro B, Miyoshi J, Takai Y, Morton CC, Maas RL, The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet. 2012 Feb;131(2):235-50
4	J:182780 Lim ST, Chang A, Giuliano RE, Federoff HJ, Ectodomain shedding of nectin-1 regulates the maintenance of dendritic spine density. J Neurochem. 2012 Mar;120(5):741-51
1	J:294113 Lough KJ, Byrd KM, Descovich CP, Spitzer DC, Bergman AJ, Beaudoin GM 3rd, Reichardt LF, Williams SE, Telophase correction refines division orientation in stratified epithelia. Elife. 2019 Dec 13;8:e49249
1*	J:226036 Maddala R, Nagendran T, Lang RA, Morozov A, Rao PV, Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
2	J:240358 Maeta K, Edamatsu H, Nishihara K, Ikutomo J, Bilasy SE, Kataoka T, Crucial Role of Rapgef2 and Rapgef6, a Family of Guanine Nucleotide Exchange Factors for Rap1 Small GTPase, in Formation of Apical Surface Adherens Junctions and Neural Progenitor Development in the Mouse Cerebral Cortex. eNeuro. 2016 May-Jun;3(3):ENEURO.0142-16.2016
1	J:204322 Majima T, Takeuchi K, Sano K, Hirashima M, Zankov DP, Tanaka-Okamoto M, Ishizaki H, Miyoshi J, Ogita H, An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo. PLoS One. 2013;8(6):e68134
2*	J:78857 Manabe N, Hirai S, Imai F, Nakanishi H, Takai Y, Ohno S, Association of ASIP/mPAR-3 with adherens junctions of mouse neuroepithelial cells. Dev Dyn. 2002 Sep;225(1):61-9
1	J:321073 Mollo MR, Antonini D, Mitchell K, Fortugno P, Costanzo A, Dixon J, Brancati F, Missero C, p63-dependent and independent mechanisms of nectin-1 and nectin-4 regulation in the epidermis. Exp Dermatol. 2015 Feb;24(2):114-9
2	J:65651 Neidhardt L, Gasca S, Wertz K, Obermayr F, Worpenberg S, Lehrach H, Herrmann BG, Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos. Mech Dev. 2000 Nov;98(1-2):77-94
16	J:90052 Okabe N, Ozaki-Kuroda K, Nakanishi H, Shimizu K, Takai Y, Expression patterns of nectins and afadin during epithelial remodeling in the mouse embryo. Dev Dyn. 2004 May;230(1):174-86
4	J:93147 Okabe N, Shimizu K, Ozaki-Kuroda K, Nakanishi H, Morimoto K, Takeuchi M, Katsumaru H, Murakami F, Takai Y, Contacts between the commissural axons and the floor plate cells are mediated by nectins. Dev Biol. 2004 Sep 15;273(2):244-56
4	J:255488 Premarathne S, Murtaza M, Matigian N, Jolly LA, Wood SA, Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors. Sci Rep. 2017 Aug 14;7(1):8109
3	J:243429 Rakotomamonjy J, Brunner M, Juschke C, Zang K, Huang EJ, Reichardt LF, Chenn A, Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia. Neural Dev. 2017 May 08;12(1):7
2	J:321506 Royer C, Sandham E, Slee E, Schneider F, Lagerholm CB, Godwin J, Veits N, Hathrell H, Zhou F, Leonavicius K, Garratt J, Narendra T, Vincent A, Jones C, Child T, Coward K, Graham C, Fritzsche M, Lu X, Srinivas S, ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis. Nat Commun. 2022 Feb 17;13(1):941
1	J:241040 Shah B, Lutter D, Tsytsyura Y, Glyvuk N, Sakakibara A, Klingauf J, Puschel AW, Rap1 GTPases Are Master Regulators of Neural Cell Polarity in the Developing Neocortex. Cereb Cortex. 2017 Feb 01;27(2):1253-1269
8	J:304680 Skarlatou S, Herent C, Toscano E, Mendes CS, Bouvier J, Zampieri N, Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection. Cell Rep. 2020 Jun 9;31(10):107741
1*	J:82002 Takekuni K, Ikeda W, Fujito T, Morimoto K, Takeuchi M, Monden M, Takai Y, Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouse. J Biol Chem. 2003 Feb 21;278(8):5497-500
1	J:144091 Tinkle CL, Pasolli HA, Stokes N, Fuchs E, New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15405-10
4*	J:65653 Wertz K, Herrmann BG, Large-scale screen for genes involved in gonad development. Mech Dev. 2000 Nov;98(1-2):51-70
1*	J:190636 Wiese CB, Ireland S, Fleming NL, Yu J, Valerius MT, Georgas K, Chiu HS, Brennan J, Armstrong J, Little MH, McMahon AP, Southard-Smith EM, A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract. Front Neurosci. 2012;6:130
2	J:229121 Yamamoto H, Mandai K, Konno D, Maruo T, Matsuzaki F, Takai Y, Impairment of radial glial scaffold-dependent neuronal migration and formation of double cortex by genetic ablation of afadin. Brain Res. 2015 Sep 16;1620:139-52
6	J:209314 Yamamoto H, Maruo T, Majima T, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Mandai K, Takai Y, Genetic deletion of afadin causes hydrocephalus by destruction of adherens junctions in radial glial and ependymal cells in the midbrain. PLoS One. 2013;8(11):e80356
1	J:195126 Yang Z, Zimmerman S, Brakeman PR, Beaudoin GM 3rd, Reichardt LF, Marciano DK, De novo lumen formation and elongation in the developing nephron: a central role for afadin in apical polarity. Development. 2013 Apr;140(8):1774-84
3	J:57046 Zhadanov AB, Provance DW Jr, Speer CA, Coffin JD, Goss D, Blixt JA, Reichert CM, Mercer JA, Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development. Curr Biol. 1999 Aug 26;9(16):880-8