Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Abca1. (This text reflects annotations as of Tuesday, May 21, 2013.)

---

Summary from NCBI RefSeq

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

---

Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Abca1
  • participates in the following biological processes:
    • cellular response to lipopolysaccharide ^[8]
    • cellular response to retinoic acid ^[8]
    • cholesterol efflux ^{[7, 10]}
    • cholesterol metabolic process ^[2]
    • phospholipid efflux ^[7]
  • performs the following molecular functions:
    • anion transmembrane transporter activity ^[1]
    • cholesterol transporter activity ^[7]
    • phospholipid transporter activity ^[7]
  • is located in the following cellular components:
    • Golgi apparatus ^[5]
    • integral to plasma membrane ^[5]
    • intracellular membrane-bounded organelle ^[5]
    • plasma membrane ^{[7, 10]}
• The gene product of Abca1 has been shown to bind to the gene products of Apoa1, Sptlc1. ^{[3, 9]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Abca1
  • participates in the following biological processes:
    • cholesterol efflux ^{[6, 7, 12]}
    • lipoprotein biosynthetic process ^{[4, 11]}
    • lipoprotein metabolic process ^[6]
    • peptide secretion ^[6]
    • phagocytosis, engulfment ^[5]
    • phospholipid efflux ^[7]
    • phospholipid translocation ^[5]
    • positive regulation of cholesterol efflux ^[13]
    • protein lipidation ^[9]
    • reverse cholesterol transport ^[14]
  • performs the following molecular functions:
    • cholesterol transporter activity ^[7]
    • phospholipid transporter activity ^[7]

---

Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Abca1:
  • participates in the following biological processes:
    • apolipoprotein A-I-mediated signaling pathway
    • cholesterol efflux
    • cholesterol homeostasis
    • cholesterol metabolic process
    • cholesterol transport
    • endosomal transport
    • G-protein coupled receptor signaling pathway
    • high-density lipoprotein particle assembly
    • interleukin-1 beta secretion
    • intracellular cholesterol transport
    • lipoprotein biosynthetic process
    • lysosome organization
    • phospholipid efflux
    • phospholipid homeostasis
    • phospholipid transport
    • platelet dense granule organization
    • positive regulation of cAMP biosynthetic process
    • regulation of Cdc42 protein signal transduction
    • reverse cholesterol transport
  • performs the following molecular functions:
    • apolipoprotein A-I binding
    • apolipoprotein A-I receptor activity
    • apolipoprotein binding
    • ATP binding
    • ATPase activity, coupled to transmembrane movement of substances
    • cholesterol transporter activity
    • phospholipid transporter activity
    • small GTPase binding
    • syntaxin binding
  • is located in the following cellular components:
    • cell surface
    • endocytic vesicle
    • high-density lipoprotein particle
    • integral to plasma membrane
    • membrane raft
    • phagocytic vesicle

---

Summary text based on GO annotations supported by structural data

• Abca1 encodes a protein or proteins that contain the following InterPro domains: AAA+ ATPase domain, ABC transporter A, ABCA, ABC transporter, conserved site, ABC transporter-like, indicating that the gene product:
  • performs the following molecular functions:
    • ATPase activity
    • nucleoside-triphosphatase activity
    • nucleotide binding

---

Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Abca1 indicates that it:
  • participates in the following biological processes:
    • transport
  • performs the following molecular functions:
    • nucleotide binding
  • is located in the following cellular components:
    • integral to membrane
    • membrane

---

References

1. Becq F et al. (1997) ABC1, an ATP binding cassette transporter required for phagocytosis of apoptotic cells, generates a regulated anion flux after expression in Xenopus laevis oocytes. J Biol Chem, 272:2695-9. (PubMed:9006906)
2. Chen W et al. (2001) Preferential ATP-binding Cassette Transporter A1-mediated Cholesterol Efflux from Late Endosomes/Lysosomes. J Biol Chem, 276:43564-9. (PubMed:11559713)
3. Coveney E et al. (1996) Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals. Surgery, 120:265-72. (PubMed:8751592)
4. Francone OL et al. (2003) Abnormal phospholipid composition impairs HDL biogenesis and maturation in mice lacking Abca1. Biochemistry, 42:8569-78. (PubMed:12859204)
5. Hamon Y et al. (2000) ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nat Cell Biol, 2:399-406. (PubMed:10878804)
6. Hirsch-Reinshagen V et al. (2004) Deficiency of ABCA1 impairs apolipoprotein E metabolism in brain. J Biol Chem, 279:41197-207. (PubMed:15269218)
7. Linsel-Nitschke P et al. (2005) Potential role of ABCA7 in cellular lipid efflux to apoA-I. J Lipid Res, 46:86-92. (PubMed:15520449)
8. Maitra U et al. (2009) An innate immunity signaling process suppresses macrophage ABCA1 expression through IRAK-1-mediated downregulation of retinoic acid receptor alpha and NFATc2. Mol Cell Biol, 29:5989-97. (PubMed:19752193)
9. Maric J et al. (2005) Intracellular lipidation of newly synthesized apolipoprotein A-I in primary murine hepatocytes. J Biol Chem, 280:39942-9. (PubMed:16204232)
10. Martinez LO et al. (2003) Phosphorylation of a pest sequence in ABCA1 promotes calpain degradation and is reversed by ApoA-I. J Biol Chem, 278:37368-74. (PubMed:12869555)
11. Tsujita M et al. (2005) On the hepatic mechanism of HDL assembly by the ABCA1/apoA-I pathway. J Lipid Res, 46:154-62. (PubMed:15520446)
12. Wang MD et al. (2007) Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. J Lipid Res, 48:633-45. (PubMed:17148552)
13. Wang N et al. (2004) ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins. Proc Natl Acad Sci U S A, 101:9774-9. (PubMed:15210959)
14. Wang X et al. (2007) Macrophage ABCA1 and ABCG1, but not SR-BI, promote macrophage reverse cholesterol transport in vivo. J Clin Invest, 117:2216-24. (PubMed:17657311)

---

---

Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

---