Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Chuk. (This text reflects annotations as of Tuesday, May 21, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Chuk
  • participates in the following biological processes:
    • protein phosphorylation ^[7]
  • performs the following molecular functions:
    • protein kinase activity ^[7]
  • is located in the following cellular components:
    • CD40 receptor complex ^[6]
    • internal side of plasma membrane ^[6]
• The gene product of Chuk has been shown to bind to the gene products of Fam92a, Ikbkb, Ikbkg, Irf7, Krt10, Terf2ip. ^{[3, 4, 5, 8, 9, 11, 12, 13]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Chuk
  • participates in the following biological processes:
    • lactation ^[1]
    • mammary gland alveolus development ^[1]
    • mammary gland epithelial cell proliferation ^[1]
    • morphogenesis of an epithelial sheet ^[10]
    • odontogenesis of dentin-containing tooth ^[10]
    • osteoclast differentiation ^[2]
    • positive regulation of I-kappaB kinase/NF-kappaB cascade ^[1]
• Researchers have inferred, based on genetic interactions, that the gene product of Chuk
  • participates in the following biological processes:
    • lactation based on interactions with Ccnd1 ^[1]
    • mammary gland alveolus development based on interactions with Ccnd1 ^[1]
    • mammary gland epithelial cell proliferation based on interactions with Ccnd1 ^[1]
    • osteoclast differentiation based on interactions with Tnf, Tnfsf11 ^[2]
    • positive regulation of I-kappaB kinase/NF-kappaB cascade based on interactions with Tnfsf11 ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Chuk:
  • participates in the following biological processes:
    • protein phosphorylation
    • response to lipopolysaccharide
    • response to organic substance
    • Rho protein signal transduction
    • striated muscle cell differentiation
  • performs the following molecular functions:
    • identical protein binding
    • IkappaB kinase activity
    • protein heterodimerization activity
    • protein kinase activity
  • is located in the following cellular components:
    • cytoplasm
    • cytosol
    • IkappaB kinase complex
    • intracellular membrane-bounded organelle
    • nucleus

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Summary text based on GO annotations supported by structural data

• Chuk encodes a protein or proteins that contain the following InterPro domains: I-kappa-kinase-beta NEMO binding domain, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine/threonine-protein kinase, active site, indicating that the gene product:
  • performs the following molecular functions:
    • ATP binding
    • protein serine/threonine kinase activity
    • transferase activity, transferring phosphorus-containing groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Chuk indicates that it:
  • participates in the following biological processes:
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • nucleotide binding
    • protein serine/threonine kinase activity
    • transferase activity

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References

1. Cao Y et al. (2001) IKKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development. Cell, 107:763-75. (PubMed:11747812)
2. Chaisson ML et al. (2004) Osteoclast differentiation is impaired in the absence of inhibitor of kappa B kinase alpha. J Biol Chem, 279:54841-8. (PubMed:15485831)
3. Chen RA et al. (2008) Inhibition of IkappaB kinase by vaccinia virus virulence factor B14. PLoS Pathog, 4:e22. (PubMed:18266467)
4. Cui J et al. (2010) NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways. Cell, 141:483-96. (PubMed:20434986)
5. Hoshino K et al. (2006) IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature, 440:949-53. (PubMed:16612387)
6. Hostager BS et al. (2010) HOIL-1L interacting protein (HOIP) as an NF-kappaB regulating component of the CD40 signaling complex. PLoS One, 5:e11380. (PubMed:20614026)
7. Makowski L et al. (2005) The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activities. J Biol Chem, 280:12888-95. (PubMed:15684432)
8. McKenzie FR et al. (2000) Functional isoforms of IkappaB kinase alpha (IKKalpha) lacking leucine zipper and helix-loop-helix domains reveal that IKKalpha and IKKbeta have different activation requirements. Mol Cell Biol, 20:2635-49. (PubMed:10733566)
9. Neely RJ et al. (2011) The RET/PTC3 oncogene activates classical NF-kappaB by stabilizing NIK. Oncogene, 30:87-96. (PubMed:20818435)
10. Ohazama A et al. (2004) A dual role for Ikk alpha in tooth development. Dev Cell, 6:219-27. (PubMed:14960276)
11. Papin J et al. (2004) Bioinformatics and cellular signaling. Curr Opin Biotechnol, 15:78-81. (PubMed:15102471)
12. Richardson RJ et al. (2006) Irf6 is a key determinant of the keratinocyte proliferation-differentiation switch. Nat Genet, 38:1329-34. (PubMed:17041603)
13. Teo H et al. (2010) Telomere-independent Rap1 is an IKK adaptor and regulates NF-kappaB-dependent gene expression. Nat Cell Biol, 12:758-67. (PubMed:20622870)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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