Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Wnt3a. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Wnt3a
  • participates in the following biological processes:
    • anterior/posterior pattern specification ^[39]
    • axon guidance ^[34]
    • axonogenesis ^[21]
    • canonical Wnt receptor signaling pathway ^{[1, 9, 12, 13, 14, 15, 16, 22, 23, 25, 29, 30, 50, 52, 53]}
    • cell proliferation in forebrain ^[51]
    • cellular protein localization ^[41]
    • extracellular matrix organization ^[3]
    • hemopoiesis ^[35]
    • mammary gland development ^[6]
    • negative regulation of axon extension involved in axon guidance ^[34]
    • negative regulation of fat cell differentiation ^{[14, 15]}
    • negative regulation of neurogenesis ^[51]
    • negative regulation of neuron projection development ^[32]
    • platelet activation ^[44]
    • platelet aggregation ^[44]
    • positive regulation of B cell proliferation ^[36]
    • positive regulation of canonical Wnt receptor signaling pathway ^[31]
    • positive regulation of canonical Wnt receptor signaling pathway involved in controlling type B pancreatic cell proliferation ^[40]
    • positive regulation of cell proliferation ^{[7, 40]}
    • positive regulation of cell-cell adhesion mediated by cadherin ^[49]
    • positive regulation of collateral sprouting in absence of injury ^[34]
    • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process ^[26]
    • positive regulation of sequence-specific DNA binding transcription factor activity ^[50]
    • positive regulation of transcription from RNA polymerase II promoter ^[20]
    • positive regulation of transcription, DNA-dependent ^{[8, 40, 50]}
    • regulation of cell differentiation ^[35]
    • signalosome assembly ^[4]
    • somatic stem cell division ^[35]
    • spinal cord association neuron differentiation ^[27]
  • performs the following molecular functions:
    • frizzled-2 binding ^[38]
    • transcription coactivator activity ^[40]
  • is located in the following cellular components:
    • cell surface ^[43]
    • extracellular space ^{[19, 21]}
• The gene product of Wnt3a has been shown to bind to the gene products of Cthrc1, Frzb, Lrp6, Ryk, Sfrp1, Sfrp2, Sfrp4. ^{[19, 21, 24, 48, 53, 57]} Researchers have inferred, based on physical interactions, that the gene product of Wnt3a
  • performs the following molecular functions:
    • frizzled binding ^[53]
    • protein domain specific binding ^[21]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Wnt3a
  • participates in the following biological processes:
    • canonical Wnt receptor signaling pathway ^[45]
    • determination of left/right symmetry ^[28]
    • dorsal/ventral neural tube patterning ^[47]
    • heart looping ^[28]
    • hippocampus development ^[18]
    • in utero embryonic development ^[11]
    • mesoderm development ^[47]
    • paraxial mesodermal cell fate commitment ^[56]
    • post-anal tail morphogenesis ^[47]
    • somitogenesis ^{[2, 28, 47]}
    • Wnt receptor signaling pathway involved in forebrain neuroblast division ^[18]
• Researchers have inferred, based on genetic interactions, that the gene product of Wnt3a
  • participates in the following biological processes:
    • axis elongation involved in somitogenesis based on interactions with Lrp6 ^[33]
    • axonogenesis based on interactions with Ryk ^[21]
    • canonical Wnt receptor signaling pathway based on interactions with Bcl9, Bcl9l, Dvl1, Dvl2, Frzb, Fzd2, Fzd4, Fzd8, Lrp6 ^{[1, 5, 10, 17, 38, 46, 55]}
    • cardiac muscle cell fate commitment based on interactions with AW551984 ^[54]
    • inner ear morphogenesis based on interactions with Wnt1 ^[37]
    • negative regulation of fat cell differentiation based on interactions with Cebpb, Cebpd ^[15]
    • osteoblast differentiation based on interactions with Cyr61 ^[42]
    • positive regulation of protein tyrosine kinase activity based on interactions with Dvl2 ^[55]
    • positive regulation of transcription from RNA polymerase II promoter based on interactions with Bcl9, Bcl9l ^[5]
    • regulation of axonogenesis based on interactions with Apc ^[34]
    • regulation of microtubule cytoskeleton organization based on interactions with Dvl1 ^[34]
    • skeletal muscle cell differentiation based on interactions with Bcl9, Bcl9l ^[5]
    • spinal cord association neuron differentiation based on interactions with Wnt1 ^[27]
    • Wnt receptor signaling pathway based on interactions with Fzd3, Fzd4, Fzd8, Lef1, Lrp6 ^{[33, 36, 46]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Wnt3a:
  • participates in the following biological processes:
    • axis specification
    • BMP signaling pathway
    • canonical Wnt receptor signaling pathway
    • canonical Wnt receptor signaling pathway involved in cardiac muscle cell fate commitment
    • convergent extension
    • embryonic pattern specification
    • hematopoietic stem cell proliferation
    • midbrain-hindbrain boundary development
    • negative regulation of heart induction by canonical Wnt receptor signaling pathway
    • negative regulation of transcription from RNA polymerase II promoter
    • notochord development
    • palate development
    • positive regulation of canonical Wnt receptor signaling pathway
    • positive regulation of cardiac muscle cell differentiation
    • positive regulation of catenin import into nucleus
    • positive regulation of dermatome development
    • positive regulation of mesodermal cell fate specification
    • positive regulation of peptidyl-serine phosphorylation
    • positive regulation of protein binding
    • positive regulation of protein phosphorylation
    • positive regulation of receptor internalization
    • positive regulation of transcription from RNA polymerase II promoter
    • Wnt receptor signaling pathway, calcium modulating pathway
  • performs the following molecular functions:
    • receptor agonist activity
  • is located in the following cellular components:
    • early endosome
    • late endosome
    • membrane raft
    • plasma membrane

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Summary text based on GO annotations supported by structural data

• Wnt3a encodes a protein or proteins that contain the following InterPro domains: Wnt, Wnt protein, conserved site, Wnt-3 protein, indicating that the gene product:
  • participates in the following biological processes:
    • multicellular organismal development
  • is located in the following cellular components:
    • extracellular region

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Wnt3a indicates that it:
  • participates in the following biological processes:
    • multicellular organismal development
  • is located in the following cellular components:
    • extracellular region
    • proteinaceous extracellular matrix

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References

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27. Muroyama Y et al. (2002) Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord. Genes Dev, 16:548-53. (PubMed:11877374)
28. Nakaya MA et al. (2005) Wnt3alinks left-right determination with segmentation and anteroposterior axis elongation. Development, 132:5425-36. (PubMed:16291790)
29. Nam JS et al. (2006) Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression. J Biol Chem, 281:13247-57. (PubMed:16543246)
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31. Ouji Y et al. (2008) Wnt-10b, uniquely among Wnts, promotes epithelial differentiation and shaft growth. Biochem Biophys Res Commun, 367:299-304. (PubMed:18155657)
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37. Riccomagno MM et al. (2005) Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh. Genes Dev, 19:1612-23. (PubMed:15961523)
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39. Satoh K et al. (2004) Anteriorization of neural fate by inhibitor of beta-catenin and T cell factor (ICAT), a negative regulator of Wnt signaling. Proc Natl Acad Sci U S A, 101:8017-21. (PubMed:15148409)
40. Schinner S et al. (2008) Regulation of insulin secretion, glucokinase gene transcription and beta cell proliferation by adipocyte-derived Wnt signalling molecules. Diabetologia, 51:147-54. (PubMed:17994217)
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42. Si W et al. (2006) CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells. Mol Cell Biol, 26:2955-64. (PubMed:16581771)
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48. Tanaka K et al. (2000) The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family. Eur J Biochem, 267:4300-11. (PubMed:10866835)
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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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