Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Trf. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Trf
  • participates in the following biological processes:
    • actin filament organization ^[5]
    • activation of JUN kinase activity ^[5]
    • ERK1 and ERK2 cascade ^[5]
    • osteoclast differentiation ^[5]
    • positive regulation of bone resorption ^[5]
    • positive regulation of cell motility ^[5]
    • positive regulation of phosphorylation ^[5]
    • positive regulation of transcription, DNA-dependent ^[5]
    • SMAD protein signal transduction ^[9]
  • is located in the following cellular components:
    • coated pit ^[7]
    • endocytic vesicle ^[3]
    • endosome ^{[2, 4, 8]}
    • recycling endosome ^{[1, 10]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Trf
  • participates in the following biological processes:
    • iron ion transport ^[6]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Trf:
  • participates in the following biological processes:
    • ferric iron transport
    • positive regulation of myelination
  • performs the following molecular functions:
    • ferric iron binding
    • ferric iron transmembrane transporter activity
  • is located in the following cellular components:
    • apical plasma membrane
    • basal part of cell
    • basal plasma membrane
    • basement membrane
    • coated pit
    • cytoplasmic membrane-bounded vesicle
    • early endosome
    • endocytic vesicle
    • extracellular space
    • late endosome
    • mitochondrion
    • perinuclear region of cytoplasm
    • recycling endosome

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Summary text based on GO annotations supported by structural data

• Trf encodes a protein or proteins that contain the following InterPro domains: Transferrin, Transferrin family, Transferrin family, iron binding site, indicating that the gene product:
  • participates in the following biological processes:
    • cellular iron ion homeostasis
  • is located in the following cellular components:
    • extracellular region

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Trf indicates that it:
  • participates in the following biological processes:
    • ion transport
    • iron ion homeostasis
    • transport
  • performs the following molecular functions:
    • metal ion binding
  • is located in the following cellular components:
    • extracellular region

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References

1. Canonne-Hergaux F et al. (2001) Characterization of the iron transporter DMT1 (NRAMP2/DCT1) in red blood cells of normal and anemic mk/mk mice. Blood, 98:3823-30. (PubMed:11739192)
2. Capurro MI et al. (2008) Glypican-3 inhibits Hedgehog signaling during development by competing with patched for Hedgehog binding. Dev Cell, 14:700-11. (PubMed:18477453)
3. Galperin E et al. (2002) EHD3: a protein that resides in recycling tubular and vesicular membrane structures and interacts with EHD1. Traffic, 3:575-89. (PubMed:12121420)
4. Guilherme A et al. (2004) Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem, 279:40062-75. (PubMed:15247266)
5. Ishii KA et al. (2009) Coordination of PGC-1beta and iron uptake in mitochondrial biogenesis and osteoclast activation. Nat Med, 15:259-66. (PubMed:19252502)
6. McKie AT et al. (2001) An iron-regulated ferric reductase associated with the absorption of dietary iron. Science, 291:1755-9. (PubMed:11230685)
7. Molla-Herman A et al. (2010) The ciliary pocket: an endocytic membrane domain at the base of primary and motile cilia. J Cell Sci, 123:1785-95. (PubMed:20427320)
8. Ohgami RS et al. (2005) Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat Genet, 37:1264-9. (PubMed:16227996)
9. Sirard C et al. (1998) The tumor suppressor gene Smad4/Dpc4 is required for gastrulation and later for anterior development of the mouse embryo. Genes Dev, 12:107-19. (PubMed:9420335)
10. Uechi Y et al. (2009) Rap2 function requires palmitoylation and recycling endosome localization. Biochem Biophys Res Commun, 378:732-7. (PubMed:19061864)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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