Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Rad51. (This text reflects annotations as of Tuesday, May 21, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Rad51
  • participates in the following biological processes:
    • double-strand break repair via homologous recombination ^[3]
    • meiosis ^{[6, 11]}
  • is located in the following cellular components:
    • condensed chromosome ^{[2, 6, 8, 11, 17]}
    • condensed nuclear chromosome ^{[7, 10, 18]}
    • lateral element ^{[4, 14]}
    • nucleus ^[9]
• The gene product of Rad51 has been shown to bind to the gene products of Brca2, Swi5. ^{[1, 3, 5, 12, 13, 16]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Rad51
  • participates in the following biological processes:
    • double-strand break repair via homologous recombination ^[15]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Rad51:
  • participates in the following biological processes:
    • DNA unwinding involved in replication
    • double-strand break repair via homologous recombination
    • positive regulation of DNA ligation
    • protein homooligomerization
  • performs the following molecular functions:
    • ATP binding
    • double-stranded DNA binding
    • identical protein binding
    • protein C-terminus binding
    • single-stranded DNA binding
    • single-stranded DNA-dependent ATPase activity
  • is located in the following cellular components:
    • cytoplasm
    • mitochondrion
    • nucleus
    • perinuclear region of cytoplasm
    • PML body

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Summary text based on GO annotations supported by structural data

• Rad51 encodes a protein or proteins that contain the following InterPro domains: AAA+ ATPase domain, DNA recombination and repair protein Rad51, C-terminal, DNA recombination and repair protein, RecA-like, DNA recombination/repair protein Rad51, DNA recombination/repair protein RecA, monomer-monomer interface, DNA recombination/repair protein RecA/RadB, ATP-binding domain, DNA repair Rad51/transcription factor NusA, alpha-helical, Helix-hairpin-helix DNA-binding motif, class 1, indicating that the gene product:
  • participates in the following biological processes:
    • DNA metabolic process
  • performs the following molecular functions:
    • damaged DNA binding
    • DNA binding
    • DNA-dependent ATPase activity
    • nucleoside-triphosphatase activity
    • nucleotide binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Rad51 indicates that it:
  • participates in the following biological processes:
    • DNA recombination
  • performs the following molecular functions:
    • DNA binding
    • nucleotide binding

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References

1. Akamatsu Y et al. (2010) Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination. PLoS Genet, 6:e1001160. (PubMed:20976249)
2. Cherry SM et al. (2007) The Mre11 complex influences DNA repair, synapsis, and crossing over in murine meiosis. Curr Biol, 17:373-8. (PubMed:17291760)
3. Gudmundsdottir K et al. (2004) DSS1 is required for RAD51 focus formation and genomic stability in mammalian cells. EMBO Rep, 5:989-93. (PubMed:15359272)
4. Herran Y et al. (2011) The cohesin subunit RAD21L functions in meiotic synapsis and exhibits sexual dimorphism in fertility. EMBO J, 30:3091-105. (PubMed:21743440)
5. Houghtaling S et al. (2003) Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout mice. Genes Dev, 17:2021-35. (PubMed:12893777)
6. Kneitz B et al. (2000) MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice. Genes Dev, 14:1085-97. (PubMed:10809667)
7. Kumar R et al. (2010) Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev, 24:1266-80. (PubMed:20551173)
8. Kwan KY et al. (2003) Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III beta. Proc Natl Acad Sci U S A, 100:2526-31. (PubMed:12591952)
9. Li MJ et al. (1996) Rad51 expression and localization in B cells carrying out class switch recombination. Proc Natl Acad Sci U S A, 93:10222-7. (PubMed:8816780)
10. Libby BJ et al. (2003) Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice. Proc Natl Acad Sci U S A, 100:15706-11. (PubMed:14668445)
11. Lipkin SM et al. (2002) Meiotic arrest and aneuploidy in MLH3-deficient mice. Nat Genet, 31:385-90. (PubMed:12091911)
12. Sarkisian CJ et al. (2001) Analysis of murine brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals. J Biol Chem, 276:37640-8. (PubMed:11477095)
13. Sharan SK et al. (1997) Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2 [see comments] Nature, 386:804-10. (PubMed:9126738)
14. Soper SF et al. (2008) Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev Cell, 15:285-97. (PubMed:18694567)
15. Stark JM et al. (2004) Genetic steps of mammalian homologous repair with distinct mutagenic consequences. Mol Cell Biol, 24:9305-16. (PubMed:15485900)
16. Suzuki H et al. (2001) Protein-protein interaction panel using mouse full-length cDNAs. Genome Res, 11:1758-65. (PubMed:11591653)
17. Wong JC et al. (2003) Targeted disruption of exons 1 to 6 of the Fanconi Anemia group A gene leads to growth retardation, strain-specific microphthalmia, meiotic defects and primordial germ cell hypoplasia. Hum Mol Genet, 12:2063-76. (PubMed:12913077)
18. Yang F et al. (2008) Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. J Cell Biol, 180:673-9. (PubMed:18283110)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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