Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pklr. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pklr
  • participates in the following biological processes:
    • phosphorylation ^{[2, 3]}
  • performs the following molecular functions:
    • pyruvate kinase activity ^{[2, 3]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pklr
  • participates in the following biological processes:
    • response to other organism ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pklr:
  • participates in the following biological processes:
    • ATP biosynthetic process
    • carbohydrate metabolic process
    • cellular response to insulin stimulus
    • glycolysis
    • pyruvate biosynthetic process
    • response to ATP
    • response to cAMP
    • response to heat
    • response to lithium ion
  • performs the following molecular functions:
    • pyruvate kinase activity
  • is located in the following cellular components:
    • cytosol

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Summary text based on GO annotations supported by structural data

• Pklr encodes a protein or proteins that contain the following InterPro domains: Pyruvate kinase, Pyruvate kinase, active site, Pyruvate kinase, alpha/beta, Pyruvate kinase, barrel, Pyruvate kinase, beta-barrel insert domain, Pyruvate kinase, C-terminal, Pyruvate kinase-like, insert domain, Pyruvate/Phosphoenolpyruvate kinase, indicating that the gene product:
  • performs the following molecular functions:
    • catalytic activity
    • magnesium ion binding
    • potassium ion binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pklr indicates that it:
  • participates in the following biological processes:
    • metabolic process
  • performs the following molecular functions:
    • ATP binding
    • catalytic activity
    • kinase activity
    • metal ion binding
    • nucleotide binding
    • transferase activity

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References

1. Min-Oo G et al. (2003) Pyruvate kinase deficiency in mice protects against malaria. Nat Genet, 35:357-62. (PubMed:14595440)
2. Peters J et al. (1981) Polymorphism of kidney pyruvate kinase in the mouse is determined by a gene, Pk-3, on chromosome 9. Biochem Genet, 19:757-69. (PubMed:7295297)
3. White RA et al. (2004) Chromosomal localization, hematologic characterization, and iron metabolism of the hereditary erythroblastic anemia (hea) mutant mouse. Blood, 104:1511-8. (PubMed:15155459)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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