Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pfkm. (This text reflects annotations as of Wednesday, January 23, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of October 6, 2008. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pfkm
  • participates in the following biological processes:
    • carbohydrate phosphorylation ^{[1, 4]}
    • glycolysis ^[1]
  • performs the following molecular functions:
    • 6-phosphofructokinase activity ^[1]
    • phosphofructokinase activity ^[4]
  • is located in the following cellular components:
    • cytosol ^[1]
• The gene product of Pfkm has been shown to bind to the gene products of Gstm5, Hk1. ^[2]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pfkm
  • participates in the following biological processes:
    • carbohydrate phosphorylation ^[3]
    • glucose homeostasis ^[3]
    • glycolysis ^[3]
    • positive regulation of insulin secretion ^[3]
  • performs the following molecular functions:
    • 6-phosphofructokinase activity ^[3]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pfkm:
  • participates in the following biological processes:
    • carbohydrate phosphorylation
    • fructose 1,6-bisphosphate metabolic process
    • fructose 6-phosphate metabolic process
    • glycolysis
    • muscle cell homeostasis
    • protein homotetramerization
    • protein oligomerization
  • performs the following molecular functions:
    • 6-phosphofructokinase activity
    • AMP binding
    • ATP binding
    • carbohydrate binding
    • fructose binding
    • fructose-6-phosphate binding
    • identical protein binding
    • kinase binding
    • phosphofructokinase activity
    • protein C-terminus binding
  • is located in the following cellular components:
    • 6-phosphofructokinase complex
    • apical plasma membrane

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Summary text based on GO annotations supported by structural data

• Pfkm encodes a protein or proteins that contain the following InterPro domains: 6-phosphofructokinase, eukaryotic type, Phosphofructokinase, Phosphofructokinase domain, Phosphofructokinase, conserved site, indicating that the gene product:
  • is located in the following cellular components:
    • cytoplasm

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pfkm indicates that it:
  • participates in the following biological processes:
    • metabolic process
    • phosphorylation
  • performs the following molecular functions:
    • catalytic activity
    • kinase activity
    • metal ion binding
    • nucleotide binding
    • transferase activity

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References

1. Gekakis N et al. (1989) Phosphofructokinase isozyme expression during myoblast differentiation. J Biol Chem, 264:3658-61. (PubMed:2521854)
2. Nakamura N et al. (2010) Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5. Biol Reprod, 82:504-15. (PubMed:19889946)
3. Richard AM et al. (2007) Tissue-dependent loss of phosphofructokinase-M in mice with interrupted activity of the distal promoter: impairment in insulin secretion. Am J Physiol Endocrinol Metab, 293:E794-801. (PubMed:17595219)
4. Schliselfeld LH et al. (2002) Inverse relationship of skeletal muscle glycogen from wild-type and genetically modified mice to their phosphorylase a activity. Biochem Biophys Res Commun, 290:874-7. (PubMed:11785984)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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