Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Notch2. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Notch2
  • participates in the following biological processes:
    • positive regulation of osteoclast differentiation ^[3]
    • wound healing ^[6]
  • is located in the following cellular components:
    • nucleus ^[6]
• The gene product of Notch2 has been shown to bind to the gene products of Dll1, Jag1, Jag2. ^{[6, 9]} Researchers have inferred, based on physical interactions, that the gene product of Notch2
  • performs the following molecular functions:
    • enzyme binding ^[2]
    • NF-kappaB binding ^[3]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Notch2
  • participates in the following biological processes:
    • determination of left/right symmetry ^[5]
    • in utero embryonic development ^[4]
    • morphogenesis of an epithelial sheet ^[7]
    • placenta blood vessel development ^[4]
    • placenta development ^[4]
• Researchers have inferred, based on genetic interactions, that the gene product of Notch2
  • participates in the following biological processes:
    • embryonic limb morphogenesis based on interactions with Notch1 ^[8]
    • heart looping based on interactions with Notch1 ^[5]
    • humoral immune response based on interactions with Notch1 ^[1]
    • inflammatory response to antigenic stimulus based on interactions with Notch1 ^[1]
    • interleukin-4 secretion based on interactions with Notch1 ^[1]
    • left/right axis specification based on interactions with Notch1 ^[5]
    • positive regulation of apoptotic process based on interactions with Notch1 ^[8]
• Based on the experimental annotations above, MGI curators have inferred, that the gene product of Notch2
  • participates in the following biological processes:
    • Notch signaling pathway ^[9]
  • is located in the following cellular components:
    • integral to plasma membrane ^[9]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Notch2:
  • participates in the following biological processes:
    • atrial septum morphogenesis
    • bone remodeling
    • cell cycle arrest
    • cell fate determination
    • cell growth
    • negative regulation of cell proliferation
    • positive regulation of Ras protein signal transduction
    • pulmonary valve morphogenesis
  • is located in the following cellular components:
    • cell surface
    • integral to plasma membrane
    • nucleus

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Summary text based on GO annotations supported by structural data

• Notch2 encodes a protein or proteins that contain the following InterPro domains: Ankyrin repeat, Ankyrin repeat-containing domain, Domain of unknown function DUF3454, notch, EGF-like calcium-binding, EGF-like calcium-binding, conserved site, EGF-like, conserved site, EGF-type aspartate/asparagine hydroxylation site, Epidermal growth factor-like domain, Neurogenic locus Notch 2, Notch, Notch domain, Notch, NOD domain, Notch, NODP domain, indicating that the gene product:
  • participates in the following biological processes:
    • cell differentiation
    • multicellular organismal development
    • regulation of developmental process
  • performs the following molecular functions:
    • calcium ion binding
  • is located in the following cellular components:
    • integral to membrane
    • membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Notch2 indicates that it:
  • participates in the following biological processes:
    • cell differentiation
    • multicellular organismal development
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent
  • is located in the following cellular components:
    • integral to membrane
    • membrane
    • plasma membrane

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References

1. Amsen D et al. (2007) Direct regulation of gata3 expression determines the T helper differentiation potential of notch. Immunity, 27:89-99. (PubMed:17658279)
2. Coleman ML et al. (2007) Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor. J Biol Chem, 282:24027-38. (PubMed:17573339)
3. Fukushima H et al. (2008) The association of Notch2 and NF-kappaB accelerates RANKL-induced osteoclastogenesis. Mol Cell Biol, 28:6402-12. (PubMed:18710934)
4. Hamada Y et al. (2007) Notch2 is required for formation of the placental circulatory system, but not for cell-type specification in the developing mouse placenta. Differentiation, 75:268-78. (PubMed:17359302)
5. Krebs LT et al. (2003) Notch signaling regulates left-right asymmetry determination by inducing Nodal expression. Genes Dev, 17:1207-12. (PubMed:12730124)
6. McCright B. (2003) Notch signaling in kidney development. Curr Opin Nephrol Hypertens, 12:5-10. (PubMed:12496659)
7. McCright B et al. (2002) A mouse model of Alagille syndrome: Notch2 as a genetic modifier of Jag1 haploinsufficiency. Development, 129:1075-82. (PubMed:11861489)
8. Pan Y et al. (2005) Notch1 and 2 cooperate in limb ectoderm to receive an early Jagged2 signal regulating interdigital apoptosis. Dev Biol, 286:472-82. (PubMed:16169548)
9. Shimizu K et al. (1999) Mouse jagged1 physically interacts with notch2 and other notch receptors. Assessment by quantitative methods. J Biol Chem, 274:32961-9. (PubMed:10551863)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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