Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Nkx3-1. (This text reflects annotations as of Tuesday, May 21, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Nkx3-1
  • participates in the following biological processes:
    • negative regulation of insulin-like growth factor receptor signaling pathway ^[5]
    • positive regulation of transcription, DNA-dependent ^[3]
  • performs the following molecular functions:
    • DNA binding ^[3]
    • MADS box domain binding ^[11]
    • sequence-specific DNA binding ^[7]
    • sequence-specific DNA binding transcription factor activity ^[3]
    • transcription factor binding ^[3]
  • is located in the following cellular components:
    • intracellular ^[8]
    • nucleus ^[4]
• The gene product of Nkx3-1 has been shown to bind to the gene products of Fem1b. ^[10] Researchers have inferred, based on physical interactions, that the gene product of Nkx3-1
  • performs the following molecular functions:
    • transcription factor binding ^[11]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Nkx3-1
  • participates in the following biological processes:
    • branching involved in prostate gland morphogenesis ^{[2, 6, 9]}
    • branching morphogenesis of an epithelial tube ^[9]
    • epithelial cell proliferation involved in salivary gland morphogenesis ^[6]
    • negative regulation of epithelial cell proliferation ^[9]
    • negative regulation of epithelial cell proliferation involved in prostate gland development ^{[1, 9]}
    • negative regulation of transcription, DNA-dependent ^[8]
    • protein kinase B signaling cascade ^[8]
    • response to testosterone stimulus ^[7]
    • salivary gland development ^[6]
  • performs the following molecular functions:
    • RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity ^[11]
• Researchers have inferred, based on genetic interactions, that the gene product of Nkx3-1
  • participates in the following biological processes:
    • negative regulation of cell proliferation based on interactions with ^[5]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Nkx3-1:
  • participates in the following biological processes:
    • activation of cysteine-type endopeptidase activity involved in apoptotic process
    • androgen receptor signaling pathway
    • cellular response to hypoxia
    • cellular response to steroid hormone stimulus
    • mitotic cell cycle arrest
    • negative regulation of cell proliferation
    • negative regulation of estrogen receptor binding
    • negative regulation of gene expression
    • negative regulation of mitotic cell cycle
    • negative regulation of transcription, DNA-dependent
    • positive regulation of androgen secretion
    • positive regulation of apoptotic signaling pathway
    • positive regulation of cell death
    • positive regulation of cell division
    • positive regulation of cell proliferation
    • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
    • positive regulation of gene expression
    • positive regulation of intrinsic apoptotic signaling pathway
    • positive regulation of mitotic cell cycle
    • positive regulation of phosphatidylinositol 3-kinase cascade
    • positive regulation of protein kinase activity
    • positive regulation of protein phosphorylation
    • positive regulation of response to DNA damage stimulus
    • positive regulation of transcription from RNA polymerase II promoter
    • positive regulation of transcription, DNA-dependent
    • protein kinase B signaling cascade
  • performs the following molecular functions:
    • androgen receptor activity
    • core promoter binding
    • cysteine-type endopeptidase activator activity involved in apoptotic process
    • estrogen receptor activity
    • estrogen receptor binding
    • histone deacetylase binding
    • protein binding transcription factor activity
    • protein kinase activator activity
    • protein self-association
    • sequence-specific DNA binding
    • sequence-specific DNA binding transcription factor activity
    • transcription factor binding
    • transcription regulatory region DNA binding
    • transcription regulatory region sequence-specific DNA binding
  • is located in the following cellular components:
    • intracellular
    • nucleus

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Summary text based on GO annotations supported by structural data

• Nkx3-1 encodes a protein or proteins that contain the following InterPro domains: Homeobox, conserved site, Homeodomain, Homeodomain, metazoa, Homeodomain-like, indicating that the gene product:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Nkx3-1 indicates that it:
  • participates in the following biological processes:
    • multicellular organismal development
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent

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References

1. Abdulkadir SA et al. (2002) Conditional loss of nkx3.1 in adult mice induces prostatic intraepithelial neoplasia. Mol Cell Biol, 22:1495-503. (PubMed:11839815)
2. Bhatia-Gaur R et al. (1999) Roles for Nkx3.1 in prostate development and cancer. Genes Dev, 13:966-77. (PubMed:10215624)
3. Carson JA et al. (2000) The smooth muscle gamma -actin gene promoter is a molecular target for the mouse bagpipe homologue, mNkx3-1, and serum response factor J Biol Chem, 275:39061-72. (PubMed:10993896)
4. Gao N et al. (2005) Forkhead box A1 regulates prostate ductal morphogenesis and promotes epithelial cell maturation. Development, 132:3431-43. (PubMed:15987773)
5. Muhlbradt E et al. (2009) NKX3.1 activates expression of insulin-like growth factor binding protein-3 to mediate insulin-like growth factor-I signaling and cell proliferation. Cancer Res, 69:2615-22. (PubMed:19258508)
6. Schneider A et al. (2000) Targeted disruption of the nkx3.1 gene in mice results in morphogenetic defects of minor salivary glands: parallels to glandular duct morphogenesis in prostate Mech Dev, 95:163-74. (PubMed:10906459)
7. Sciavolino PJ et al. (1997) Tissue-specific expression of murine Nkx3.1 in the male urogenital system. Dev Dyn, 209:127-38. (PubMed:9142502)
8. Song H et al. (2009) Loss of Nkx3.1 leads to the activation of discrete downstream target genes during prostate tumorigenesis. Oncogene, 28:3307-19. (PubMed:19597465)
9. Tanaka M et al. (2000) Nkx3.1, a murine homolog of drosophila bagpipe, regulates epithelial ductal branching and proliferation of the prostate and palatine glands Dev Dyn, 219:248-60. (PubMed:11002344)
10. Wang X et al. (2008) Mouse Fem1b interacts with the Nkx3.1 homeoprotein and is required for proper male secondary sexual development. Dev Dyn, 237:2963-2972. (PubMed:18816836)
11. Zhang Y et al. (2008) Structural and functional analysis of domains mediating interaction between the bagpipe homologue, Nkx3.1 and serum response factor. Exp Biol Med (Maywood), 233:297-309. (PubMed:18296735)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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