Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Ldha. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Two transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, Sep 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Ldha
  • participates in the following biological processes:
    • cellular response to extracellular stimulus ^[3]
    • oxidation-reduction process ^[7]
  • performs the following molecular functions:
    • L-lactate dehydrogenase activity ^[7]
  • is located in the following cellular components:
    • cytosol ^[6]
    • flagellum ^[2]
    • mitochondrion ^[4]
• The gene product of Ldha has been shown to bind to the gene products of Hnrnpd. ^[5]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Ldha
  • participates in the following biological processes:
    • oxidation-reduction process ^[1]
  • performs the following molecular functions:
    • L-lactate dehydrogenase activity ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Ldha:
  • participates in the following biological processes:
    • lactate metabolic process
    • NAD metabolic process
    • oxidation-reduction process
    • positive regulation of apoptotic process
  • performs the following molecular functions:
    • identical protein binding
    • kinase binding
    • L-lactate dehydrogenase activity
    • lactate dehydrogenase activity
    • NAD binding

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Summary text based on GO annotations supported by structural data

• Ldha encodes a protein or proteins that contain the following InterPro domains: L-lactate dehydrogenase, L-lactate dehydrogenase, active site, L-lactate/malate dehydrogenase, Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal, Lactate/malate dehydrogenase, C-terminal, Lactate/malate dehydrogenase, N-terminal, NAD(P)-binding domain, indicating that the gene product:
  • participates in the following biological processes:
    • carbohydrate metabolic process
    • cellular carbohydrate metabolic process
    • glycolysis
  • performs the following molecular functions:
    • catalytic activity
    • nucleotide binding
    • oxidoreductase activity
    • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
  • is located in the following cellular components:
    • cytoplasm

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Ldha indicates that it:
  • participates in the following biological processes:
    • glycolysis
  • performs the following molecular functions:
    • oxidoreductase activity
  • is located in the following cellular components:
    • cytoplasm

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References

1. Charles DJ et al. (1987) Linear dose-response relationship of erythrocyte enzyme-activity mutations in offspring of ethylnitrosourea-treated mice. Mutat Res, 176:81-91. (PubMed:3796661)
2. Krisfalusi M et al. (2006) Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Biol Reprod, 75:270-8. (PubMed:16687649)
3. Leimeister C et al. (1999) Screen for genes regulated during early kidney morphogenesis. Dev Genet, 24:273-83. (PubMed:10322635)
4. Pagliarini DJ et al. (2008) A mitochondrial protein compendium elucidates complex I disease biology. Cell, 134:112-23. (PubMed:18614015)
5. Pioli PA et al. (2002) Lactate dehydrogenase is an AU-rich element-binding protein that directly interacts with AUF1. J Biol Chem, 277:35738-45. (PubMed:12107167)
6. Putcha GV et al. (2002) Intrinsic and extrinsic pathway signaling during neuronal apoptosis: lessons from the analysis of mutant mice. J Cell Biol, 157:441-53. (PubMed:11980919)
7. Stallings RL et al. (1981) Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7. Somatic Cell Genet, 7:683-98. (PubMed:7323947)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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