Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Itk. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Itk
  • is located in the following cellular components:
    • cell-cell junction ^[3]
• The gene product of Itk has been shown to bind to the gene products of Themis. ^[2]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Itk
  • participates in the following biological processes:
    • activation of phospholipase C activity ^[4]
    • adaptive immune response ^[4]
    • cytokine production ^[4]
    • interferon-gamma production ^[1]
    • interleukin-4 production ^[1]
    • NK T cell differentiation ^[1]
    • T cell receptor signaling pathway ^[4]
• Researchers have inferred, based on genetic interactions, that the gene product of Itk
  • participates in the following biological processes:
    • interferon-gamma production based on interactions with Txk ^[1]
    • interleukin-4 production based on interactions with Txk ^[1]
    • NK T cell differentiation based on interactions with Txk ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Itk:
  • participates in the following biological processes:
    • peptidyl-tyrosine phosphorylation
  • performs the following molecular functions:
    • non-membrane spanning protein tyrosine kinase activity

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Summary text based on GO annotations supported by structural data

• Itk encodes a protein or proteins that contain the following InterPro domains: Pleckstrin homology domain, Pleckstrin homology-like domain, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine-threonine/tyrosine-protein kinase catalytic domain, SH2 domain, Src homology-3 domain, Tyrosine-protein kinase, active site, Tyrosine-protein kinase, catalytic domain, Zinc finger, Btk motif, indicating that the gene product:
  • participates in the following biological processes:
    • intracellular signal transduction
    • protein phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • protein kinase activity
    • protein tyrosine kinase activity
    • transferase activity, transferring phosphorus-containing groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Itk indicates that it:
  • participates in the following biological processes:
    • immune system process
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • metal ion binding
    • nucleotide binding
    • protein tyrosine kinase activity
    • transferase activity
  • is located in the following cellular components:
    • cytoplasm

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References

1. Felices M et al. (2008) The tec kinases itk and rlk regulate NKT cell maturation, cytokine production, and survival. J Immunol, 180:3007-18. (PubMed:18292523)
2. Fu G et al. (2009) Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat Immunol, 10:848-56. (PubMed:19597499)
3. Liang Y et al. (2013) The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells. Nat Immunol, 14:858-66. (PubMed:23793062)
4. Schaeffer EM et al. (1999) Requirement for Tec kinases Rlk and Itk in T cell receptor signaling and immunity. Science, 284:638-41. (PubMed:10213685)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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