Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Itgam. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Itgam
  • participates in the following biological processes:
    • microglia development ^[16]
  • performs the following molecular functions:
    • heparan sulfate proteoglycan binding ^[3]
    • heparin binding ^[3]
    • opsonin binding ^[2]
  • is located in the following cellular components:
    • cell surface ^[25]
    • external side of plasma membrane ^{[2, 3, 6, 7, 10, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23]}
    • integral component of membrane ^[11]
    • nucleus ^[8]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Itgam
  • participates in the following biological processes:
    • activated T cell proliferation ^[24]
    • cell adhesion ^[4]
    • cellular extravasation ^[4]
    • leukocyte cell-cell adhesion ^[1]
    • leukocyte migration involved in inflammatory response ^[5]
    • neutrophil chemotaxis ^[4]
  • performs the following molecular functions:
    • opsonin binding ^[2]
• Researchers have inferred, based on genetic interactions, that the gene product of Itgam
  • participates in the following biological processes:
    • cell adhesion based on interactions with Itgb2, Jam3 ^[26]
    • single organismal cell-cell adhesion based on interactions with Itgb2, Jam3 ^[9]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Itgam:
  • performs the following molecular functions:
    • glycoprotein binding
    • protein heterodimerization activity
  • is located in the following cellular components:
    • cell surface
    • extracellular vesicular exosome

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Summary text based on GO annotations supported by structural data

• Itgam encodes a protein or proteins that contain the following InterPro domains: FG-GAP repeat, Integrin alpha beta-propellor, Integrin alpha chain, Integrin alpha chain, C-terminal cytoplasmic region, conserved site, Integrin alpha-2, Ribosomal protein L29, von Willebrand factor, type A, indicating that the gene product:
  • is located in the following cellular components:
    • integrin complex

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Itgam indicates that it:
  • participates in the following biological processes:
    • integrin-mediated signaling pathway
  • performs the following molecular functions:
    • metal ion binding
  • is located in the following cellular components:
    • membrane

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References

1. Aigner S et al. (1995) Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol, 7:1557-65. (PubMed:8562500)
2. Brown GD et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages. J Exp Med, 196:407-12. (PubMed:12163569)
3. Coombe DR et al. (1994) Mac-1 (CD11b/CD18) and CD45 mediate the adhesion of hematopoietic progenitor cells to stromal cell elements via recognition of stromal heparan sulfate. Blood, 84:739-52. (PubMed:8043862)
4. Ding ZM et al. (1999) Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration. J Immunol, 163:5029-38. (PubMed:10528208)
5. Ghasemzadeh M et al. (2013) The CXCR1/2 ligand NAP-2 promotes directed intravascular leukocyte migration through platelet thrombi. Blood, 121:4555-66. (PubMed:23550035)
6. Hazenbos WL et al. (1996) Impaired IgG-dependent anaphylaxis and Arthus reaction in Fc gamma RIII (CD16) deficient mice. Immunity, 5:181-8. (PubMed:8769481)
7. Kina T et al. (2000) The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol, 109:280-7. (PubMed:10848813)
8. Kramerova I et al. (2004) Null mutation of calpain 3 (p94) in mice causes abnormal sarcomere formation in vivo and in vitro. Hum Mol Genet, 13:1373-88. (PubMed:15138196)
9. Lamagna C et al. (2005) Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion. Mol Biol Cell, 16:4992-5003. (PubMed:16093349)
10. Li T et al. (1996) Distinct patterns of Fas cell surface expression during development of T- or B-lymphocyte lineages in normal, scid, and mutant mice lacking or overexpressing p53, bcl-2, or rag-2 genes. Cell Growth Differ, 7:107-14. (PubMed:8788039)
11. Manitz MP et al. (2003) Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro. Mol Cell Biol, 23:1034-43. (PubMed:12529407)
12. Marie JC et al. (2006) Cellular mechanisms of fatal early-onset autoimmunity in mice with the T cell-specific targeting of transforming growth factor-beta receptor. Immunity, 25:441-54. (PubMed:16973387)
13. Matsuzaki J et al. (2003) Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo. Cell Immunol, 224:98-105. (PubMed:14609575)
14. Nagamachi A et al. (2013) Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7. Cancer Cell, 24:305-17. (PubMed:24029230)
15. O'Connell RM et al. (2010) MicroRNAs enriched in hematopoietic stem cells differentially regulate long-term hematopoietic output. Proc Natl Acad Sci U S A, 107:14235-40. (PubMed:20660734)
16. Parenti R et al. (2010) Dynamic expression of Cx47 in mouse brain development and in the cuprizone model of myelin plasticity. Glia, 58:1594-609. (PubMed:20578039)
17. Park H et al. (2008) A point mutation in the murine Hem1 gene reveals an essential role for Hematopoietic Protein 1 in lymphopoiesis and innate immunity. J Exp Med, 205:2899-913. (PubMed:19015308)
18. Rupec RA et al. (2005) Stroma-mediated dysregulation of myelopoiesis in mice lacking I kappa B alpha. Immunity, 22:479-91. (PubMed:15845452)
19. Ruppert M et al. (1995) The L1 adhesion molecule is a cellular ligand for VLA-5. J Cell Biol, 131:1881-91. (PubMed:8557754)
20. Serrano AG et al. (2010) Contrasting effects of Sox17- and Sox18-sustained expression at the onset of blood specification. Blood, 115:3895-8. (PubMed:20228271)
21. Takai T et al. (1996) Augmented humoral and anaphylactic responses in Fc gamma RII-deficient mice. Nature, 379:346-9. (PubMed:8552190)
22. Terauchi M et al. (2009) T lymphocytes amplify the anabolic activity of parathyroid hormone through Wnt10b signaling. Cell Metab, 10:229-40. (PubMed:19723499)
23. Winkler IG et al. (2005) Serine protease inhibitors serpina1 and serpina3 are down-regulated in bone marrow during hematopoietic progenitor mobilization. J Exp Med, 201:1077-88. (PubMed:15795238)
24. Wu H et al. (2004) Deficiency of CD11b or CD11d results in reduced staphylococcal enterotoxin-induced T cell response and T cell phenotypic changes. J Immunol, 173:297-306. (PubMed:15210787)
25. Yoder MC et al. (1997) Characterization of definitive lymphohematopoietic stem cells in the day 9 murine yolk sac. Immunity, 7:335-44. (PubMed:9324354)
26. Zimmerli C et al. (2009) Adaptive immune response in JAM-C-deficient mice: normal initiation but reduced IgG memory. J Immunol, 182:4728-36. (PubMed:19342649)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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