Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Il3. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Il3
  • participates in the following biological processes:
    • cytokine-mediated signaling pathway ^[6]
    • myeloid cell apoptotic process ^[1]
    • negative regulation of apoptotic process ^{[7, 10, 17]}
    • negative regulation of autophagy ^[8]
    • negative regulation of mast cell apoptotic process ^[14]
    • positive regulation of cell proliferation ^{[3, 10, 11]}
    • positive regulation of JNK cascade ^[17]
    • positive regulation of mast cell proliferation ^{[4, 14]}
    • positive regulation of peptidyl-tyrosine phosphorylation ^{[5, 6]}
    • positive regulation of protein phosphorylation ^[17]
    • regulation of apoptotic process ^{[2, 13]}
    • regulation of gene expression ^[15]
    • regulation of protein dephosphorylation ^[2]
  • performs the following molecular functions:
    • cytokine activity ^{[3, 5, 6, 7, 10, 11, 14, 17]}
    • growth factor activity ^{[3, 14]}
  • is located in the following cellular components:
    • extracellular space ^[12]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Il3
  • participates in the following biological processes:
    • positive regulation of JNK cascade ^[17]
• Researchers have inferred, based on genetic interactions, that the gene product of Il3
  • participates in the following biological processes:
    • positive regulation of cell proliferation based on interactions with Kitl ^[12]
    • positive regulation of myeloid leukocyte differentiation based on interactions with Kitl ^{[12, 16]}
    • regulation of glycolysis based on interactions with Hif1a ^[9]
    • response to hypoxia based on interactions with Hif1a ^[9]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Il3:
  • participates in the following biological processes:
    • embryonic hemopoiesis
    • hemopoiesis
    • positive regulation of cell proliferation
    • positive regulation of DNA replication
    • positive regulation of tyrosine phosphorylation of Stat5 protein
    • regulation of cell growth
    • response to hormone stimulus
  • performs the following molecular functions:
    • cytokine activity
    • interleukin-3 receptor binding
  • is located in the following cellular components:
    • extracellular space

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Summary text based on GO annotations supported by structural data

• Il3 encodes a protein or proteins that contain the following InterPro domains: Four-helical cytokine, core, Four-helical cytokine-like, core, Interleukin-3, indicating that the gene product:
  • participates in the following biological processes:
    • immune response
  • is located in the following cellular components:
    • extracellular region

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Il3 indicates that it:
  • is located in the following cellular components:
    • extracellular region

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References

1. Barge RM et al. (1997) Differential involvement of caspases in apoptosis of myeloid leukemic cells induced by chemotherapy versus growth factor withdrawal. FEBS Lett, 409:207-10. (PubMed:9202146)
2. Chiang CW et al. (2003) Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis. Mol Cell Biol, 23:6350-62. (PubMed:12944463)
3. Hilton DJ et al. (1994) Cloning of a murine IL-11 receptor alpha-chain; requirement for gp130 for high affinity binding and signal transduction. EMBO J, 13:4765-75. (PubMed:7957045)
4. Ikeda K et al. (2005) Stat5a is essential for the proliferation and survival of murine mast cells. Int Arch Allergy Immunol, 137 Suppl 1:45-50. (PubMed:15947484)
5. Iseki M et al. (2000) Molecular cloning of the mouse APS as a member of the Lnk family adaptor proteins. Biochem Biophys Res Commun, 272:45-54. (PubMed:10872802)
6. Ito T et al. (1994) Interleukin 3 stimulates protein synthesis by regulating double-stranded RNA-dependent protein kinase. Proc Natl Acad Sci U S A, 91:7455-9. (PubMed:7519779)
7. Kieslinger M et al. (2000) Antiapoptotic activity of Stat5 required during terminal stages of myeloid differentiation. Genes Dev, 14:232-44. (PubMed:10652277)
8. Lum JJ et al. (2005) Growth factor regulation of autophagy and cell survival in the absence of apoptosis. Cell, 120:237-48. (PubMed:15680329)
9. Lum JJ et al. (2007) The transcription factor HIF-1{alpha} plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev, 21:1037-49. (PubMed:17437992)
10. Miyazaki T et al. (1995) Three distinct IL-2 signaling pathways mediated by bcl-2, c-myc, and lck cooperate in hematopoietic cell proliferation. Cell, 81:223-31. (PubMed:7736574)
11. Okada S et al. (1992) Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors. Exp Hematol, 20:546-51. (PubMed:1350248)
12. Ploemacher RE et al. (1993) Interleukin-12 enhances interleukin-3 dependent multilineage hematopoietic colony formation stimulated by interleukin-11 or steel factor. Leukemia, 7:1374-80. (PubMed:7690439)
13. Seo SY et al. (2004) BAD is a pro-survival factor prior to activation of its pro-apoptotic function. J Biol Chem, 279:42240-9. (PubMed:15231831)
14. Shelburne CP et al. (2003) Stat5 expression is critical for mast cell development and survival. Blood, 102:1290-7. (PubMed:12714518)
15. Traver D et al. (1998) Mice defective in two apoptosis pathways in the myeloid lineage develop acute myeloblastic leukemia. Immunity, 9:47-57. (PubMed:9697835)
16. Tsuji K et al. (1992) Enhancement of murine hematopoiesis by synergistic interactions between steel factor (ligand for c-kit), interleukin-11, and other early acting factors in culture. Blood, 79:2855-60. (PubMed:1375116)
17. Yu C et al. (2004) JNK suppresses apoptosis via phosphorylation of the proapoptotic Bcl-2 family protein BAD. Mol Cell, 13:329-40. (PubMed:14967141)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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