Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Gnai2. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only two are known so far. [provided by RefSeq, Oct 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• The gene product of Gnai2 has been shown to bind to the gene products of Ptger3. ^[3] Researchers have inferred, based on physical interactions, that the gene product of Gnai2
  • participates in the following biological processes:
    • cell proliferation ^[5]
  • performs the following molecular functions:
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Gnai2
  • participates in the following biological processes:
    • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway ^[4]
    • G-protein coupled acetylcholine receptor signaling pathway ^[1]
• Researchers have inferred, based on genetic interactions, that the gene product of Gnai2
  • participates in the following biological processes:
    • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway based on interactions with Drd2 ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Gnai2:
  • participates in the following biological processes:
    • activation of MAPKK activity
    • adenosine receptor signaling pathway
    • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
    • cell division
    • G-protein coupled receptor signaling pathway
    • gamma-aminobutyric acid signaling pathway
    • intracellular protein kinase cascade
    • negative regulation of synaptic transmission
    • positive regulation of cell proliferation
    • regulation of calcium ion transport
  • performs the following molecular functions:
    • G-protein beta/gamma-subunit complex binding
    • G-protein coupled receptor binding
    • GTP binding
    • GTPase activity
    • signal transducer activity
  • is located in the following cellular components:
    • centrosome
    • cytoplasm
    • cytosol
    • extrinsic to internal side of plasma membrane
    • heterotrimeric G-protein complex
    • membrane
    • membrane raft
    • midbody
    • nucleus
    • plasma membrane

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Summary text based on GO annotations supported by structural data

• Gnai2 encodes a protein or proteins that contain the following InterPro domains: G protein alpha subunit, helical insertion, G-protein alpha subunit, group I, Guanine nucleotide binding protein (G-protein), alpha subunit, indicating that the gene product:
  • participates in the following biological processes:
    • adenylate cyclase-modulating G-protein coupled receptor signaling pathway
    • signal transduction
  • performs the following molecular functions:
    • guanyl nucleotide binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Gnai2 indicates that it:
  • participates in the following biological processes:
    • cell cycle
    • signal transduction
  • performs the following molecular functions:
    • metal ion binding
    • nucleotide binding
  • is located in the following cellular components:
    • cytoskeleton

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References

1. Chen F et al. (2001) Lack of muscarinic regulation of Ca(2+) channels in G(i2)alpha gene knockout mouse hearts. Am J Physiol Heart Circ Physiol, 280:H1989-95. (PubMed:11299198)
2. Guiramand J et al. (1995) Alternative splicing of the dopamine D2 receptor directs specificity of coupling to G-proteins. J Biol Chem, 270:7354-8. (PubMed:7706278)
3. Irie A et al. (1994) The C-terminus of the prostaglandin-E-receptor EP3 subtype is essential for activation of GTP-binding protein. Eur J Biochem, 224:161-6. (PubMed:8076637)
4. Rudolph U et al. (1996) Adenylyl cyclase inhibition and altered G protein subunit expression and ADP-ribosylation patterns in tissues and cells from Gi2 alpha-/-mice. Proc Natl Acad Sci U S A, 93:3209-14. (PubMed:8622915)
5. Weissman JT et al. (2004) G-protein-coupled receptor-mediated activation of rap GTPases: characterization of a novel Galphai regulated pathway. Oncogene, 23:241-9. (PubMed:14712229)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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