Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Slc2a4. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Slc2a4
  • participates in the following biological processes:
    • amylopectin biosynthetic process ^[4]
    • brown fat cell differentiation ^[17]
    • cellular response to insulin stimulus ^[16]
    • cellular response to osmotic stress ^[16]
    • glucose transport ^{[1, 7]}
    • hexose transmembrane transport ^[7]
  • performs the following molecular functions:
    • glucose transmembrane transporter activity ^[7]
  • is located in the following cellular components:
    • cytoplasmic vesicle membrane ^[5]
    • endosome ^[8]
    • extracellular vesicular exosome ^[2]
    • insulin-responsive compartment ^[16]
    • integral to membrane ^[4]
    • integral to plasma membrane ^[4]
    • membrane ^[6]
    • perinuclear region of cytoplasm ^{[3, 10]}
    • plasma membrane ^{[3, 5, 8, 9, 10, 11, 12, 13, 14, 15]}
    • sarcolemma ^[6]
    • trans-Golgi network transport vesicle ^[9]
• The gene product of Slc2a4 has been shown to bind to the gene products of Flot1. ^[6]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Slc2a4:
  • participates in the following biological processes:
    • cellular response to insulin stimulus
    • glucose homeostasis
    • glucose transport
  • performs the following molecular functions:
    • D-glucose transmembrane transporter activity
    • glucose transmembrane transporter activity
  • is located in the following cellular components:
    • clathrin-coated vesicle
    • coated pit
    • endosome
    • external side of plasma membrane
    • insulin-responsive compartment
    • intracellular membrane-bounded organelle
    • membrane
    • multivesicular body
    • perinuclear region of cytoplasm
    • plasma membrane
    • sarcolemma
    • vesicle
    • vesicle membrane

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Summary text based on GO annotations supported by structural data

• Slc2a4 encodes a protein or proteins that contain the following InterPro domains: General substrate transporter, Glucose transporter, type 4 (GLUT4), Major facilitator superfamily domain, Major facilitator superfamily domain, general substrate transporter, Sugar transporter, conserved site, Sugar/inositol transporter, indicating that the gene product:
  • participates in the following biological processes:
    • transmembrane transport
    • transport
  • performs the following molecular functions:
    • substrate-specific transmembrane transporter activity
    • transmembrane transporter activity
    • transporter activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Slc2a4 indicates that it:
  • participates in the following biological processes:
    • carbohydrate transport
    • transport
  • is located in the following cellular components:
    • cytoplasm

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References

1. Barnes BR et al. (2005) 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics. FASEB J, 19:773-9. (PubMed:15857891)
2. Blanc L et al. (2009) The water channel aquaporin-1 partitions into exosomes during reticulocyte maturation: implication for the regulation of cell volume. Blood, 114:3928-34. (PubMed:19724054)
3. Bogan JS et al. (1999) Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4. J Cell Biol, 146:609-20. (PubMed:10444069)
4. Bogan JS et al. (2003) Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking. Nature, 425:727-33. (PubMed:14562105)
5. Bose A et al. (2002) Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c. Nature, 420:821-4. (PubMed:12490950)
6. Fecchi K et al. (2006) Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells. FASEB J, 20:705-7. (PubMed:16455755)
7. Gray S et al. (2002) The Kruppel-like factor KLF15 regulates the insulin-sensitive glucose transporter GLUT4. J Biol Chem, 277:34322-8. (PubMed:12097321)
8. Guilherme A et al. (2004) Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem, 279:40062-75. (PubMed:15247266)
9. Hatanaka T et al. (2006) Regulation of amino acid transporter ATA2 by ubiquitin ligase Nedd4-2. J Biol Chem, 281:35922-30. (PubMed:17003038)
10. Hosaka T et al. (2005) p115 Interacts with the GLUT4 vesicle protein, IRAP, and plays a critical role in insulin-stimulated GLUT4 translocation. Mol Biol Cell, 16:2882-90. (PubMed:15800058)
11. Macaulay SL et al. (2002) Cellular munc18c levels can modulate glucose transport rate and GLUT4 translocation in 3T3L1 cells. FEBS Lett, 528:154-60. (PubMed:12297296)
12. MaCaulay SL et al. (2003) Insulin stimulates movement of sorting nexin 9 between cellular compartments: a putative role mediating cell surface receptor expression and insulin action. Biochem J, 376:123-34. (PubMed:12917015)
13. Schwenk RW et al. (2010) Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes. Diabetologia, 53:2209-19. (PubMed:20582536)
14. Taguchi A et al. (2008) Identification of Glypican3 as a novel GLUT4-binding protein. Biochem Biophys Res Commun, 369:1204-8. (PubMed:18343214)
15. Widberg CH et al. (2003) Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation. J Biol Chem, 278:35093-101. (PubMed:12832401)
16. Williams D et al. (2008) Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes. J Cell Biol, 180:375-87. (PubMed:18227281)
17. Wu Y et al. (2008) Wdnm1-like, a new adipokine with a role in MMP-2 activation. Am J Physiol Endocrinol Metab, 295:E205-15. (PubMed:18492766)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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