Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Gfap. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Gfap
  • is located in the following cellular components:
    • cell body ^{[14, 15]}
    • cell part ^[18]
    • cell projection ^[5]
    • cytoplasm ^{[1, 2, 8, 9, 11]}
    • intermediate filament ^{[4, 13, 16, 17, 19]}
    • membrane ^[10]
• The gene product of Gfap has been shown to bind to the gene products of Synm. ^[7]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Gfap
  • participates in the following biological processes:
    • Bergmann glial cell differentiation ^[3]
    • extracellular matrix organization ^[13]
    • intermediate filament organization ^[12]
    • intermediate filament-based process ^[20]
    • long-term synaptic potentiation ^[12]
    • negative regulation of neuron projection development ^[13]
    • neuron projection regeneration ^[21]
    • positive regulation of Schwann cell proliferation ^[21]
    • regulation of neurotransmitter uptake ^[6]
  • performs the following molecular functions:
    • structural molecule activity ^[20]
• Researchers have inferred, based on genetic interactions, that the gene product of Gfap
  • participates in the following biological processes:
    • astrocyte development based on interactions with Vim ^[13]
    • intermediate filament organization based on interactions with Vim ^[13]
    • negative regulation of neuron projection development based on interactions with Vim ^[13]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Gfap:
  • participates in the following biological processes:
    • positive regulation of glial cell proliferation
    • response to wounding
  • performs the following molecular functions:
    • integrin binding
    • kinase binding
    • structural constituent of cytoskeleton
  • is located in the following cellular components:
    • cytoplasm

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References

1. Eiraku M et al. (2005) DNER acts as a neuron-specific Notch ligand during Bergmann glial development. Nat Neurosci, 8:873-80. (PubMed:15965470)
2. Fukami S et al. (2002) Abeta-degrading endopeptidase, neprilysin, in mouse brain: synaptic and axonal localization inversely correlating with Abeta pathology. Neurosci Res, 43:39-56. (PubMed:12074840)
3. Gimenez Y Ribotta M et al. (2000) Comparative anatomy of the cerebellar cortex in mice lacking vimentin, GFAP, and both vimentin and GFAP. Glia, 31:69-83. (PubMed:10816608)
4. Gingras H et al. (2005) Biochemical characterization of the mammalian Cux2 protein. Gene, 344:273-85. (PubMed:15656993)
5. Ha BK et al. (2000) Localization of gp130 in the developing and adult mouse cerebellum. J Chem Neuroanat, 19:129-41. (PubMed:10989258)
6. Hughes EG et al. (2004) Loss of glial fibrillary acidic protein results in decreased glutamate transport and inhibition of PKA-induced EAAT2 cell surface trafficking. Brain Res Mol Brain Res, 124:114-23. (PubMed:15135219)
7. Jing R et al. (2007) Synemin is expressed in reactive astrocytes in neurotrauma and interacts differentially with vimentin and GFAP intermediate filament networks. J Cell Sci, 120:1267-77. (PubMed:17356066)
8. Kinoshita A et al. (2000) Differential localization of septins in the mouse brain J Comp Neurol, 428:223-39. (PubMed:11064363)
9. Kittappa R et al. (2007) The foxa2 gene controls the birth and spontaneous degeneration of dopamine neurons in old age. PLoS Biol, 5:e325. (PubMed:18076286)
10. Kleene R et al. (2001) The neural recognition molecule L1 is a sialic acid-binding lectin for CD24, which induces promotion and inhibition of neurite outgrowth. J Biol Chem, 276:21656-63. (PubMed:11283023)
11. Kuramochi Y et al. (2002) Characterization of mouse homolog of brain acyl-CoA hydrolase: molecular cloning and neuronal localization. Brain Res Mol Brain Res, 98:81-92. (PubMed:11834298)
12. McCall MA et al. (1996) Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A, 93:6361-6. (PubMed:8692820)
13. Menet V et al. (2001) Inactivation of the glial fibrillary acidic protein gene, but not that of vimentin, improves neuronal survival and neurite growth by modifying adhesion molecule expression. J Neurosci, 21:6147-58. (PubMed:11487638)
14. Molthagen M et al. (1996) Apoptotic cell death of photoreceptor cells in mice deficient for the adhesion molecule on glia (AMOG, the beta 2- subunit of the Na, K-ATPase). J Neurocytol, 25:243-55. (PubMed:8793730)
15. Parenti R et al. (2010) Dynamic expression of Cx47 in mouse brain development and in the cuprizone model of myelin plasticity. Glia, 58:1594-609. (PubMed:20578039)
16. Reuss B et al. (2003) Functions of fibroblast growth factor (FGF)-2 and FGF-5 in astroglial differentiation and blood-brain barrier permeability: evidence from mouse mutants. J Neurosci, 23:6404-12. (PubMed:12878680)
17. Sakai K et al. (2003) Neutral amino acid transporter ASCT1 is preferentially expressed in L-Ser-synthetic/storing glial cells in the mouse brain with transient expression in developing capillaries. J Neurosci, 23:550-60. (PubMed:12533615)
18. Seandel M et al. (2007) Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature, 449:346-50. (PubMed:17882221)
19. Shibata T et al. (1997) Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord. J Neurosci, 17:9212-9. (PubMed:9364068)
20. Takemura M et al. (2002) Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice. J Neurosci, 22:6972-9. (PubMed:12177195)
21. Triolo D et al. (2006) Loss of glial fibrillary acidic protein (GFAP) impairs Schwann cell proliferation and delays nerve regeneration after damage. J Cell Sci, 119:3981-93. (PubMed:16988027)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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