Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Epha2. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Epha2
  • participates in the following biological processes:
    • ephrin receptor signaling pathway ^[6]
    • lens fiber cell morphogenesis ^[2]
    • neuron differentiation ^[1]
    • osteoclast differentiation ^[4]
    • regulation of angiogenesis ^[3]
    • regulation of blood vessel endothelial cell migration ^[3]
• The gene product of Epha2 has been shown to bind to the gene products of Epha4, Vav2, Vav3. ^{[3, 8]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Epha2
  • participates in the following biological processes:
    • axial mesoderm formation ^[5]
    • blood vessel development ^[5]
    • bone remodeling ^[4]
    • branching involved in mammary gland duct morphogenesis ^[7]
    • ephrin receptor signaling pathway ^[2]
    • mammary gland epithelial cell proliferation ^[7]
    • neural tube development ^[5]
    • neuron differentiation ^[1]
    • notochord cell development ^[5]
    • notochord formation ^[5]
    • notochord morphogenesis ^[5]
    • osteoblast differentiation ^[4]
    • post-anal tail morphogenesis ^[5]
    • skeletal system development ^[5]
    • vasculogenesis ^[5]
• Researchers have inferred, based on genetic interactions, that the gene product of Epha2
  • participates in the following biological processes:
    • notochord formation based on interactions with Efna1 ^[5]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Epha2:
  • participates in the following biological processes:
    • activation of Rac GTPase activity
    • cell chemotaxis
    • cell migration
    • ephrin receptor signaling pathway
    • intrinsic apoptotic signaling pathway in response to DNA damage
    • keratinocyte differentiation
    • negative regulation of protein kinase B signaling
    • peptidyl-tyrosine phosphorylation
    • positive regulation of establishment of protein localization to plasma membrane
    • protein kinase B signaling
    • regulation of cell adhesion mediated by integrin
    • regulation of ERK1 and ERK2 cascade
    • regulation of lamellipodium assembly
    • response to growth factor
  • performs the following molecular functions:
    • transmembrane receptor protein tyrosine kinase activity
  • is located in the following cellular components:
    • focal adhesion
    • integral component of plasma membrane
    • intracellular
    • leading edge membrane
    • plasma membrane

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Summary text based on GO annotations supported by structural data

• Epha2 encodes a protein or proteins that contain the following InterPro domains: Ephrin receptor ligand binding domain, Fibronectin, type III, Galactose-binding domain-like, Immunoglobulin-like fold, Insulin-like growth factor binding protein, N-terminal, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine-threonine/tyrosine-protein kinase catalytic domain, Sterile alpha motif domain, Sterile alpha motif, type 1, Sterile alpha motif/pointed domain, Tyrosine-protein kinase, active site, Tyrosine-protein kinase, catalytic domain, Tyrosine-protein kinase, ephrin receptor, Tyrosine-protein kinase, receptor class V, conserved site, indicating that the gene product:
  • participates in the following biological processes:
    • protein phosphorylation
    • transmembrane receptor protein tyrosine kinase signaling pathway
  • performs the following molecular functions:
    • ATP binding
    • ephrin receptor activity
    • protein kinase activity
    • protein tyrosine kinase activity
    • transferase activity, transferring phosphorus-containing groups
  • is located in the following cellular components:
    • integral component of membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Epha2 indicates that it:
  • participates in the following biological processes:
    • angiogenesis
    • apoptotic process
    • cell adhesion
    • cell differentiation
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • nucleotide binding
    • protein tyrosine kinase activity
    • transferase activity
  • is located in the following cellular components:
    • cell junction
    • cell projection
    • integral component of membrane
    • membrane

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References

1. Aoki M et al. (2004) EphA receptors direct the differentiation of mammalian neural precursor cells through a mitogen-activated protein kinase-dependent pathway. J Biol Chem, 279:32643-50. (PubMed:15145949)
2. Cooper MA et al. (2008) Loss of ephrin-A5 function disrupts lens fiber cell packing and leads to cataract. Proc Natl Acad Sci U S A, 105:16620-5. (PubMed:18948590)
3. Hunter SG et al. (2006) Essential role of Vav family guanine nucleotide exchange factors in EphA receptor-mediated angiogenesis. Mol Cell Biol, 26:4830-42. (PubMed:16782872)
4. Irie N et al. (2009) Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis. J Biol Chem, 284:14637-44. (PubMed:19299512)
5. Naruse-Nakajima C et al. (2001) Involvement of EphA2 in the formation of the tail notochord via interaction with ephrinA1. Mech Dev, 102:95-105. (PubMed:11287184)
6. Tanaka M et al. (2004) Tiam1 mediates neurite outgrowth induced by ephrin-B1 and EphA2. EMBO J, 23:1075-88. (PubMed:14988728)
7. Vaught D et al. (2009) Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol Biol Cell, 20:2572-81. (PubMed:19321667)
8. Wiesner S et al. (2006) A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases. EMBO J, 25:4686-96. (PubMed:16977320)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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