Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Stx4a. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Stx4a
  • is located in the following cellular components:
    • basolateral plasma membrane ^[7]
    • cytoplasm ^[8]
    • lateral loop ^[5]
    • myelin sheath adaxonal region ^[5]
    • perinuclear region of cytoplasm ^[6]
    • plasma membrane ^[8]
    • trans-Golgi network ^[6]
• The gene product of Stx4a has been shown to bind to the gene products of Doc2b, Stxbp3a, Vamp2. ^{[1, 2, 3, 4, 6, 8]} Researchers have inferred, based on physical interactions, that the gene product of Stx4a
  • performs the following molecular functions:
    • SNARE binding ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Stx4a:
  • participates in the following biological processes:
    • cellular membrane fusion
    • protein complex assembly
    • synaptic vesicle docking involved in exocytosis
  • performs the following molecular functions:
    • Rab GTPase binding
    • SNARE binding
    • spectrin binding
  • is located in the following cellular components:
    • apical plasma membrane
    • basolateral plasma membrane
    • cell surface
    • plasma membrane
    • sarcolemma
    • SNARE complex
    • specific granule

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Summary text based on GO annotations supported by structural data

• Stx4a encodes a protein or proteins that contain the following InterPro domains: Syntaxin, N-terminal, Syntaxin/epimorphin, conserved site, t-SNARE, Target SNARE coiled-coil domain, indicating that the gene product:
  • participates in the following biological processes:
    • intracellular protein transport
    • vesicle-mediated transport
  • performs the following molecular functions:
    • SNAP receptor activity
  • is located in the following cellular components:
    • membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Stx4a indicates that it:
  • participates in the following biological processes:
    • neurotransmitter transport
    • transport
  • is located in the following cellular components:
    • integral to membrane
    • membrane

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References

1. Fukuda N et al. (2009) DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes. Diabetes, 58:377-84. (PubMed:19033398)
2. Ke B et al. (2007) Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis. J Biol Chem, 282:21786-97. (PubMed:17548353)
3. Macaulay SL et al. (2002) Cellular munc18c levels can modulate glucose transport rate and GLUT4 translocation in 3T3L1 cells. FEBS Lett, 528:154-60. (PubMed:12297296)
4. Oh E et al. (2009) Munc18c depletion selectively impairs the sustained phase of insulin release. Diabetes, 58:1165-74. (PubMed:19188424)
5. Ozcelik M et al. (2010) Pals1 is a major regulator of the epithelial-like polarization and the extension of the myelin sheath in peripheral nerves. J Neurosci, 30:4120-31. (PubMed:20237282)
6. Pooley RD et al. (2008) Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport. J Cell Sci, 121:3413-21. (PubMed:18827011)
7. Procino G et al. (2008) AQP2 exocytosis in the renal collecting duct -- involvement of SNARE isoforms and the regulatory role of Munc18b. J Cell Sci, 121:2097-106. (PubMed:18505797)
8. Widberg CH et al. (2003) Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation. J Biol Chem, 278:35093-101. (PubMed:12832401)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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