Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Braf. (This text reflects annotations as of Tuesday, May 21, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Braf
  • participates in the following biological processes:
    • protein phosphorylation ^[3]
    • response to epidermal growth factor stimulus ^[3]
  • performs the following molecular functions:
    • protein kinase activity ^[3]
  • is located in the following cellular components:
    • cytoplasm ^[4]
    • plasma membrane ^[4]
• The gene product of Braf has been shown to bind to the gene products of Raf1. ^{[1, 2]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Braf:
  • participates in the following biological processes:
    • activation of MAPKK activity
    • cellular response to calcium ion
    • MAPK cascade
    • negative regulation of apoptotic process
    • negative regulation of neuron apoptotic process
    • positive regulation of ERK1 and ERK2 cascade
    • positive regulation of gene expression
    • positive regulation of peptidyl-serine phosphorylation
    • protein heterooligomerization
    • protein phosphorylation
    • response to cAMP
    • response to epidermal growth factor stimulus
    • response to peptide hormone stimulus
  • performs the following molecular functions:
    • calcium ion binding
    • MAP kinase kinase kinase activity
    • mitogen-activated protein kinase kinase binding
    • protein heterodimerization activity
    • protein kinase activity
    • protein serine/threonine kinase activity
  • is located in the following cellular components:
    • membrane
    • neuron projection
    • nucleus

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Summary text based on GO annotations supported by structural data

• Braf encodes a protein or proteins that contain the following InterPro domains: Diacylglycerol/phorbol-ester binding, Protein kinase C-like, phorbol ester/diacylglycerol binding, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Raf-like Ras-binding, Serine-threonine/tyrosine-protein kinase catalytic domain, Serine/threonine-protein kinase, active site, indicating that the gene product:
  • participates in the following biological processes:
    • intracellular signal transduction
    • signal transduction
  • performs the following molecular functions:
    • ATP binding
    • receptor signaling protein activity
    • transferase activity, transferring phosphorus-containing groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Braf indicates that it:
  • participates in the following biological processes:
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • metal ion binding
    • nucleotide binding
    • transferase activity

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References

1. Heidorn SJ et al. (2010) Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF. Cell, 140:209-21. (PubMed:20141835)
2. Packer LM et al. (2011) Nilotinib and MEK inhibitors induce synthetic lethality through paradoxical activation of RAF in drug-resistant chronic myeloid leukemia. Cancer Cell, 20:715-27. (PubMed:22169110)
3. Ren JG et al. (2007) IQGAP1 modulates activation of B-Raf. Proc Natl Acad Sci U S A, 104:10465-9. (PubMed:17563371)
4. Xie Y et al. (2005) Six post-implantation lethal knockouts of genes for lipophilic MAPK pathway proteins are expressed in preimplantation mouse embryos and trophoblast stem cells. Mol Reprod Dev, 71:1-11. (PubMed:15736129)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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