Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Acat1. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Acat1
  • participates in the following biological processes:
    • metabolic process ^{[2, 5]}
  • performs the following molecular functions:
    • acetyl-CoA C-acetyltransferase activity ^{[2, 5]}
  • is located in the following cellular components:
    • mitochondrial inner membrane ^[1]
    • mitochondrion ^{[3, 4]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Acat1:
  • participates in the following biological processes:
    • protein homooligomerization
  • performs the following molecular functions:
    • acetyl-CoA C-acetyltransferase activity
    • coenzyme binding
    • enzyme binding
    • protein homodimerization activity
  • is located in the following cellular components:
    • mitochondrial matrix
    • mitochondrion
    • peroxisome

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Summary text based on GO annotations supported by structural data

• Acat1 encodes a protein or proteins that contain the following InterPro domains: Thiolase, Thiolase, active site, Thiolase, acyl-enzyme intermediate active site, Thiolase, C-terminal, Thiolase, conserved site, Thiolase, N-terminal, Thiolase-like, Thiolase-like, subgroup, indicating that the gene product:
  • performs the following molecular functions:
    • catalytic activity
    • transferase activity, transferring acyl groups other than amino-acyl groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Acat1 indicates that it:
  • performs the following molecular functions:
    • metal ion binding
    • transferase activity
    • transferase activity, transferring acyl groups

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References

1. Da Cruz S et al. (2003) Proteomic analysis of the mouse liver mitochondrial inner membrane. J Biol Chem, 278:41566-71. (PubMed:12865426)
2. Hyogo H et al. (2002) Leptin Promotes Biliary Cholesterol Elimination during Weight Loss in ob/ob Mice by Regulating the Enterohepatic Circulation of Bile Salts. J Biol Chem, 277:34117-24. (PubMed:12114517)
3. Mootha VK et al. (2003) Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell, 115:629-40. (PubMed:14651853)
4. Pagliarini DJ et al. (2008) A mitochondrial protein compendium elucidates complex I disease biology. Cell, 134:112-23. (PubMed:18614015)
5. Uelmen PJ et al. (1995) Tissue-specific expression and cholesterol regulation of acylcoenzyme A:cholesterol acyltransferase (ACAT) in mice. Molecular cloning of mouse ACAT cDNA, chromosomal localization, and regulation of ACAT in vivo and in vitro. J Biol Chem, 270:26192-201. (PubMed:7592824)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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