Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Shank2. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain, and a proline-rich region. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. Two alternative splice variants, encoding distinct isoforms, are reported. Additional splice variants exist but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Shank2
  • is located in the following cellular components:
    • dendritic spine ^[3]
    • ionotropic glutamate receptor complex ^[3]
    • neurofilament ^[1]
    • neuron projection ^[4]
    • photoreceptor inner segment ^[1]
    • photoreceptor outer segment ^[1]
    • plasma membrane ^[3]
    • postsynaptic membrane ^[3]
• Researchers have inferred, based on physical interactions, that the gene product of Shank2
  • performs the following molecular functions:
    • ionotropic glutamate receptor binding ^{[2, 3]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Shank2:
  • performs the following molecular functions:
    • protein C-terminus binding
    • SH3 domain binding
  • is located in the following cellular components:
    • cilium membrane
    • growth cone
    • neuronal cell body
    • postsynaptic density

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Shank2 indicates that it:
  • is located in the following cellular components:
    • cell junction
    • cell projection
    • cytoplasm
    • membrane
    • synapse

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References

1. Kim JH et al. (2009) Shank 2 expression coincides with neuronal differentiation in the developing retina. Exp Mol Med, 41:236-42. (PubMed:19299912)
2. Uchino S et al. (2006) Direct interaction of post-synaptic density-95/Dlg/ZO-1 domain-containing synaptic molecule Shank3 with GluR1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor. J Neurochem, 97:1203-14. (PubMed:16606358)
3. Uemura T et al. (2004) Direct interaction of GluRdelta2 with Shank scaffold proteins in cerebellar Purkinje cells. Mol Cell Neurosci, 26:330-41. (PubMed:15207857)
4. Verpelli C et al. (2011) Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. J Biol Chem, 286:34839-50. (PubMed:21795692)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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