Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Spred2. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Spred2
  • participates in the following biological processes:
    • negative regulation of peptidyl-threonine phosphorylation ^[3]
    • negative regulation of protein serine/threonine kinase activity ^[3]
    • positive regulation of DNA damage response, signal transduction by p53 class mediator ^[3]
    • regulation of protein deacetylation ^[3]
  • performs the following molecular functions:
    • protein serine/threonine kinase inhibitor activity ^[3]
  • is located in the following cellular components:
    • cytoplasmic vesicle ^[1]
    • plasma membrane ^[2]
• The gene product of Spred2 has been shown to bind to the gene products of Kit. ^{[2, 4]} Researchers have inferred, based on physical interactions, that the gene product of Spred2
  • performs the following molecular functions:
    • protein kinase binding ^[3]
    • stem cell factor receptor binding ^[2]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Spred2
  • participates in the following biological processes:
    • inactivation of MAPK activity ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Spred2:
  • participates in the following biological processes:
    • negative regulation of peptidyl-threonine phosphorylation
  • performs the following molecular functions:
    • protein kinase binding
  • is located in the following cellular components:
    • cytoplasm
    • nucleus

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Summary text based on GO annotations supported by structural data

• Spred2 encodes a protein or proteins that contain the following InterPro domains: c-Kit-binding domain, EVH1, Pleckstrin homology-like domain, Sprouty, indicating that the gene product:
  • participates in the following biological processes:
    • multicellular organismal development
    • regulation of signal transduction
  • is located in the following cellular components:
    • membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Spred2 indicates that it:
  • is located in the following cellular components:
    • membrane

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References

1. Engelhardt CM et al. (2004) Expression and subcellular localization of Spred proteins in mouse and human tissues. Histochem Cell Biol, 122:527-38. (PubMed:15580519)
2. Kato R et al. (2003) Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem Biophys Res Commun, 302:767-72. (PubMed:12646235)
3. Li D et al. (2010) Direct association of Sprouty-related protein with an EVH1 domain (SPRED) 1 or SPRED2 with DYRK1A modifies substrate/kinase interactions. J Biol Chem, 285:35374-85. (PubMed:20736167)
4. Mardakheh FK et al. (2009) Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling. J Cell Biol, 187:265-77. (PubMed:19822672)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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