Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pus1. (This text reflects annotations as of Wednesday, January 23, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of February 9, 2010. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pus1
  • participates in the following biological processes:
    • positive regulation of transcription from RNA polymerase II promoter ^[3]
    • tRNA pseudouridine synthesis ^{[1, 2, 3]}
  • performs the following molecular functions:
    • DNA binding ^[3]
    • ligand-dependent nuclear receptor transcription coactivator activity ^[3]
    • pseudouridine synthase activity ^[2]
  • is located in the following cellular components:
    • nucleolus ^[3]
    • nucleus ^[3]
    • transcription factor complex ^[3]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pus1
  • participates in the following biological processes:
    • metabolic process ^[3]
  • performs the following molecular functions:
    • pseudouridine synthase activity ^[3]

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Summary text based on GO annotations supported by structural data

• Pus1 encodes a protein or proteins that contain the following InterPro domains: Pseudouridine synthase I, TruA, Pseudouridine synthase I, TruA, alpha/beta domain, Pseudouridine synthase I, TruA, C-terminal, Pseudouridine synthase I, TruA, N-terminal, Pseudouridine synthase, catalytic domain, indicating that the gene product:
  • participates in the following biological processes:
    • pseudouridine synthesis
    • RNA modification
  • performs the following molecular functions:
    • RNA binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pus1 indicates that it:
  • participates in the following biological processes:
    • tRNA processing
  • performs the following molecular functions:
    • isomerase activity
  • is located in the following cellular components:
    • mitochondrion

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References

1. Chen J et al. (2000) Mouse pseudouridine synthase 1: gene structure and alternative splicing of pre-mRNA Biochem J, 352 Pt 2:465-73. (PubMed:11085940)
2. Chen J et al. (2000) Pseudouridine synthase 3 from mouse modifies the anticodon loop of tRNA. Biochemistry, 39:12723-30. (PubMed:11027153)
3. Zhao X et al. (2004) Regulation of nuclear receptor activity by a pseudouridine synthase through posttranscriptional modification of steroid receptor RNA activator. Mol Cell, 15:549-58. (PubMed:15327771)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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