Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Rnaseh2a. (This text reflects annotations as of Thursday, July 24, 2014.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of February 9, 2010. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Rnaseh2a
  • is located in the following cellular components:
    • ribonuclease H2 complex ^[2]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Rnaseh2a
  • participates in the following biological processes:
    • mismatch repair ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Rnaseh2a:
  • participates in the following biological processes:
    • mismatch repair
    • RNA catabolic process
    • RNA phosphodiester bond hydrolysis, endonucleolytic
  • performs the following molecular functions:
    • RNA-DNA hybrid ribonuclease activity
  • is located in the following cellular components:
    • ribonuclease H2 complex

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Summary text based on GO annotations supported by structural data

• Rnaseh2a encodes a protein or proteins that contain the following InterPro domains: Ribonuclease H-like domain, Ribonuclease H2, subunit A, Ribonuclease HII, helix-loop-helix cap domain, Ribonuclease HII/HIII, Ribonuclease HII/HIII domain, indicating that the gene product:
  • participates in the following biological processes:
    • RNA metabolic process
  • performs the following molecular functions:
    • nucleic acid binding
    • RNA binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Rnaseh2a indicates that it:
  • performs the following molecular functions:
    • endonuclease activity
    • hydrolase activity
    • metal ion binding
    • nuclease activity
  • is located in the following cellular components:
    • nucleus
• Rnaseh2a has been associated with the Enzyme Commission numbers: 3.1.26.-, 3.1.26.4, which implies that the gene product:
  • performs the following molecular functions:
    • bidentate ribonuclease III activity

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References

1. Ghodgaonkar MM et al. (2013) Ribonucleotides misincorporated into DNA act as strand-discrimination signals in eukaryotic mismatch repair. Mol Cell, 50:323-32. (PubMed:23603115)
2. Shaban NM et al. (2010) The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction. J Biol Chem, 285:3617-24. (PubMed:19923215)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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