Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Stub1. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Stub1
  • participates in the following biological processes:
    • protein polyubiquitination ^[1]
  • performs the following molecular functions:
    • protein homodimerization activity ^[4]
    • ubiquitin-ubiquitin ligase activity ^[1]
• The gene product of Stub1 has been shown to bind to the gene products of Ilk. ^{[2, 4]} Researchers have inferred, based on physical interactions, that the gene product of Stub1
  • performs the following molecular functions:
    • ubiquitin protein ligase binding ^[3]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Stub1
  • participates in the following biological processes:
    • cellular response to misfolded protein ^[3]
    • misfolded or incompletely synthesized protein catabolic process ^[3]
    • proteasome-mediated ubiquitin-dependent protein catabolic process ^[3]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Stub1:
  • participates in the following biological processes:
    • cellular response to misfolded protein
    • misfolded or incompletely synthesized protein catabolic process
    • positive regulation of chaperone-mediated protein complex assembly
    • positive regulation of proteasomal ubiquitin-dependent protein catabolic process
    • positive regulation of protein ubiquitination
    • proteasome-mediated ubiquitin-dependent protein catabolic process
    • protein autoubiquitination
    • protein K63-linked ubiquitination
    • protein polyubiquitination
    • regulation of glucocorticoid metabolic process
    • ubiquitin-dependent protein catabolic process
    • ubiquitin-dependent SMAD protein catabolic process
  • performs the following molecular functions:
    • enzyme binding
    • Hsp70 protein binding
    • Hsp90 protein binding
    • kinase binding
    • misfolded protein binding
    • SMAD binding
    • TPR domain binding
    • ubiquitin-protein transferase activity
  • is located in the following cellular components:
    • cytoplasm
    • extracellular vesicular exosome
    • intermediate filament cytoskeleton
    • nuclear inclusion body
    • nucleus
    • plasma membrane
    • ubiquitin ligase complex

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Stub1 indicates that it:
  • participates in the following biological processes:
    • cellular response to DNA damage stimulus
    • DNA repair
  • performs the following molecular functions:
    • ligase activity
• Stub1 has been associated with the Enzyme Commission numbers: 6.3.2.-, which implies that the gene product:
  • performs the following molecular functions:
    • coenzyme F420-0 gamma-glutamyl ligase activity
    • coenzyme F420-2 alpha-glutamyl ligase activity
    • protein-glutamic acid ligase activity
    • protein-glycine ligase activity
    • protein-glycine ligase activity, elongating
    • protein-glycine ligase activity, initiating
    • ribosomal S6-glutamic acid ligase activity
    • tubulin-glutamic acid ligase activity
    • tubulin-glycine ligase activity
    • UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity

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References

1. Hatakeyama S et al. (2001) U box proteins as a new family of ubiquitin-protein ligases. J Biol Chem, 276:33111-20. (PubMed:11435423)
2. Radovanac K et al. (2013) Stabilization of integrin-linked kinase by the Hsp90-CHIP axis impacts cellular force generation, migration and the fibrotic response. EMBO J, 32:1409-24. (PubMed:23612611)
3. Scaglione KM et al. (2011) Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell, 43:599-612. (PubMed:21855799)
4. Zhang M et al. (2005) Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex. Mol Cell, 20:525-38. (PubMed:16307917)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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