Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Klf1. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Klf1
  • participates in the following biological processes:
    • chromatin remodeling ^[2]
    • positive regulation of transcription from RNA polymerase II promoter ^[5]
    • positive regulation of transcription, DNA-dependent ^[5]
• Researchers have inferred, based on physical interactions, that the gene product of Klf1
  • performs the following molecular functions:
    • activating transcription factor binding ^[5]
    • protein domain specific binding ^[5]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Klf1
  • participates in the following biological processes:
    • embryonic hemopoiesis ^{[3, 6]}
    • erythrocyte development ^{[1, 3]}
    • erythrocyte maturation ^[4]
    • in utero embryonic development ^[3]
    • liver development ^[3]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Klf1:
  • participates in the following biological processes:
    • erythrocyte differentiation
    • positive regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • core promoter proximal region sequence-specific DNA binding
    • DNA binding
    • transcription regulatory region DNA binding
  • is located in the following cellular components:
    • nuclear chromatin
    • nucleus

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Summary text based on GO annotations supported by structural data

• Klf1 encodes a protein or proteins that contain the following InterPro domains: Zinc finger C2H2-type/integrase DNA-binding domain, Zinc finger, C2H2, Zinc finger, C2H2-like, indicating that the gene product:
  • performs the following molecular functions:
    • nucleic acid binding
    • zinc ion binding
  • is located in the following cellular components:
    • intracellular

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Klf1 indicates that it:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent
  • performs the following molecular functions:
    • metal ion binding

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References

1. Drissen R et al. (2005) The erythroid phenotype of EKLF-null mice: defects in hemoglobin metabolism and membrane stability. Mol Cell Biol, 25:5205-14. (PubMed:15923635)
2. Drissen R et al. (2004) The active spatial organization of the beta-globin locus requires the transcription factor EKLF. Genes Dev, 18:2485-90. (PubMed:15489291)
3. Nuez B et al. (1995) Defective haematopoiesis in fetal liver resulting from inactivation of the EKLF gene. Nature, 375:316-8. (PubMed:7753194)
4. Perkins AC et al. (1995) Lethal beta-thalassaemia in mice lacking the erythroid CACCC-transcription factor EKLF. Nature, 375:318-22. (PubMed:7753195)
5. Sengupta T et al. (2009) Distinct modes of gene regulation by a cell-specific transcriptional activator. Proc Natl Acad Sci U S A, 106:4213-8. (PubMed:19251649)
6. White RA et al. (2009) Hematologic characterization and chromosomal localization of the novel dominantly inherited mouse hemolytic anemia, neonatal anemia (Nan). Blood Cells Mol Dis, 43:141-8. (PubMed:19409822)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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