Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pdpk1. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pdpk1
  • participates in the following biological processes:
    • hyperosmotic response ^[6]
    • protein phosphorylation ^[2]
  • performs the following molecular functions:
    • 3-phosphoinositide-dependent protein kinase activity ^[2]
  • is located in the following cellular components:
    • cytoplasm ^[6]
    • cytoplasmic membrane-bounded vesicle ^[6]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pdpk1
  • participates in the following biological processes:
    • negative regulation of cardiac muscle cell apoptotic process ^[3]
    • negative regulation of toll-like receptor signaling pathway ^[1]
    • regulation of endothelial cell migration ^[4]
    • regulation of mast cell degranulation ^[5]
    • type B pancreatic cell development ^[7]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pdpk1:
  • participates in the following biological processes:
    • activation of protein kinase B activity
    • cellular response to insulin stimulus
    • focal adhesion assembly
    • negative regulation of protein kinase activity
    • negative regulation of transforming growth factor beta receptor signaling pathway
    • peptidyl-threonine phosphorylation
    • positive regulation of establishment of protein localization to plasma membrane
    • protein phosphorylation
    • regulation of apoptotic process
    • regulation of I-kappaB kinase/NF-kappaB cascade
  • performs the following molecular functions:
    • 3-phosphoinositide-dependent protein kinase activity
    • insulin receptor binding
    • protein kinase binding
    • protein serine/threonine kinase activity
  • is located in the following cellular components:
    • cytoplasm
    • cytosol
    • plasma membrane

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Summary text based on GO annotations supported by structural data

• Pdpk1 encodes a protein or proteins that contain the following InterPro domains: Pleckstrin homology-like domain, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine/threonine- / dual-specificity protein kinase, catalytic domain, Serine/threonine-protein kinase, active site, indicating that the gene product:
  • performs the following molecular functions:
    • ATP binding
    • protein kinase activity
    • transferase activity, transferring phosphorus-containing groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pdpk1 indicates that it:
  • participates in the following biological processes:
    • phosphorylation
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • nucleotide binding
    • transferase activity
  • is located in the following cellular components:
    • cell junction
    • membrane
    • nucleus

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References

1. Chaurasia B et al. (2010) Phosphoinositide-dependent kinase 1 provides negative feedback inhibition to Toll-like receptor-mediated NF-kappaB activation in macrophages. Mol Cell Biol, 30:4354-66. (PubMed:20584979)
2. Dong LQ et al. (1999) Primary structure, tissue distribution, and expression of mouse phosphoinositide-dependent protein kinase-1, a protein kinase that phosphorylates and activates protein kinase Czeta. J Biol Chem, 274:8117-22. (PubMed:10075713)
3. Ito K et al. (2009) PDK1 coordinates survival pathways and beta-adrenergic response in the heart. Proc Natl Acad Sci U S A, 106:8689-94. (PubMed:19429709)
4. Primo L et al. (2007) Essential role of PDK1 in regulating endothelial cell migration. J Cell Biol, 176:1035-47. (PubMed:17371830)
5. Shumilina E et al. (2010) Phosphoinositide-dependent kinase PDK1 in the regulation of Ca2+ entry into mast cells. Cell Physiol Biochem, 26:699-706. (PubMed:21063107)
6. Torbett NE et al. (2003) Hyperosmotic-induced protein kinase N 1 activation in a vesicular compartment is dependent upon Rac1 and 3-phosphoinositide-dependent kinase 1. J Biol Chem, 278:32344-51. (PubMed:12783890)
7. Westmoreland JJ et al. (2009) Pdk1 activity controls proliferation, survival, and growth of developing pancreatic cells. Dev Biol, 334:285-98. (PubMed:19635472)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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