Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Cldn1. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The knockout mice lacking this gene die soon after birth as a consequence of dehydration from trandermal water loss, indicating that this gene is indispensable for creating and maintaining the epidermal barrier. The protein encoded by this gene also has gastric tumor suppressive activity, and is a key factor for hepatitis C virus (HCV) entry. [provided by RefSeq, Aug 2010]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Cldn1
  • participates in the following biological processes:
    • calcium-independent cell-cell adhesion ^[5]
  • is located in the following cellular components:
    • apical plasma membrane ^[8]
    • plasma membrane ^[9]
    • tight junction ^{[2, 6, 7, 9]}
• The gene product of Cldn1 has been shown to bind to the gene products of Cldn3, Tjp1, Tjp2, Tjp3. ^{[1, 3, 4]} Researchers have inferred, based on physical interactions, that the gene product of Cldn1
  • performs the following molecular functions:
    • identical protein binding ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Cldn1:
  • participates in the following biological processes:
    • cell-cell junction organization
  • is located in the following cellular components:
    • lateral plasma membrane
    • tight junction

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Summary text based on GO annotations supported by structural data

• Cldn1 encodes a protein or proteins that contain the following InterPro domains: Claudin, Claudin, conserved site, Claudin-1, PMP-22/EMP/MP20/Claudin, indicating that the gene product:
  • performs the following molecular functions:
    • structural molecule activity
  • is located in the following cellular components:
    • membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Cldn1 indicates that it:
  • is located in the following cellular components:
    • cell junction
    • membrane

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References

1. Furuse M et al. (1999) Manner of interaction of heterogeneous claudin species within and between tight junction strands. J Cell Biol, 147:891-903. (PubMed:10562289)
2. Furuse M et al. (1998) Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol, 141:1539-50. (PubMed:9647647)
3. Hamazaki Y et al. (2002) Multi-PDZ domain protein 1 (MUPP1) is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule. J Biol Chem, 277:455-61. (PubMed:11689568)
4. Itoh M et al. (1999) Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J Cell Biol, 147:1351-63. (PubMed:10601346)
5. Kubota K et al. (1999) Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. Curr Biol, 9:1035-8. (PubMed:10508613)
6. Nico B et al. (2003) Severe alterations of endothelial and glial cells in the blood-brain barrier of dystrophic mdx mice. Glia, 42:235-51. (PubMed:12673830)
7. Ohta H et al. (2006) Developmental changes in the expression of tight junction protein claudins in murine metanephroi and embryonic kidneys. J Vet Med Sci, 68:149-55. (PubMed:16520537)
8. Plante I et al. (2010) Milk secretion and ejection are impaired in the mammary gland of mice harboring a Cx43 mutant while expression and localization of tight and adherens junction proteins remain unchanged. Biol Reprod, 82:837-47. (PubMed:20089884)
9. Tunggal JA et al. (2005) E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions. EMBO J, 24:1146-56. (PubMed:15775979)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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