Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pik3ca. (This text reflects annotations as of Thursday, January 16, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pik3ca
  • participates in the following biological processes:
    • hypomethylation of CpG island ^[8]
    • negative regulation of fibroblast apoptotic process ^[1]
    • phosphatidylinositol-3-phosphate biosynthetic process ^[3]
    • positive regulation of peptidyl-serine phosphorylation ^[8]
    • positive regulation of protein kinase activity ^[8]
    • protein phosphorylation ^[1]
    • regulation of genetic imprinting ^[8]
  • performs the following molecular functions:
    • 1-phosphatidylinositol-3-kinase activity ^[3]
    • protein kinase activator activity ^[8]
  • is located in the following cellular components:
    • lamellipodium ^[3]
• The gene product of Pik3ca has been shown to bind to the gene products of Pik3r1. ^{[4, 6, 7]} Researchers have inferred, based on physical interactions, that the gene product of Pik3ca
  • performs the following molecular functions:
    • insulin receptor substrate binding ^[5]
  • is located in the following cellular components:
    • phosphatidylinositol 3-kinase complex ^[7]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pik3ca
  • participates in the following biological processes:
    • glucose metabolic process ^[2]
    • negative regulation of neuron apoptotic process ^[9]
    • protein kinase B signaling ^[2]
    • regulation of multicellular organism growth ^[2]
  • is located in the following cellular components:
    • intracellular ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pik3ca:
  • participates in the following biological processes:
    • negative regulation of anoikis
    • phosphatidylinositol phosphorylation
    • phosphatidylinositol-3-phosphate biosynthetic process
  • performs the following molecular functions:
    • 1-phosphatidylinositol-3-kinase activity
    • 1-phosphatidylinositol-4-phosphate 3-kinase activity
    • phosphatidylinositol-4,5-bisphosphate 3-kinase activity
  • is located in the following cellular components:
    • 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
    • phosphatidylinositol 3-kinase complex
    • plasma membrane

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Summary text based on GO annotations supported by structural data

• Pik3ca encodes a protein or proteins that contain the following InterPro domains: Armadillo-type fold, C2 calcium-dependent membrane targeting, Phosphatidylinositol 3-/4-kinase, catalytic domain, Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domain, Phosphatidylinositol 3-kinase C2 (PI3K C2) domain, Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domain, Phosphatidylinositol 3/4-kinase, conserved site, Phosphatidylinositol Kinase, Phosphoinositide 3-kinase, accessory (PIK) domain, Protein kinase-like domain, indicating that the gene product:
  • participates in the following biological processes:
    • phosphatidylinositol-mediated signaling
  • performs the following molecular functions:
    • phosphotransferase activity, alcohol group as acceptor
    • transferase activity, transferring phosphorus-containing groups

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pik3ca indicates that it:
  • participates in the following biological processes:
    • angiogenesis
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • nucleotide binding
    • protein serine/threonine kinase activity
    • transferase activity

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References

1. Baki L et al. (2004) PS1 activates PI3K thus inhibiting GSK-3 activity and tau overphosphorylation: effects of FAD mutations. EMBO J, 23:2586-96. (PubMed:15192701)
2. Foukas LC et al. (2006) Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature, 441:366-70. (PubMed:16625210)
3. Gavard J et al. (2004) Lamellipodium extension and cadherin adhesion: two cell responses to cadherin activation relying on distinct signalling pathways. J Cell Sci, 117:257-70. (PubMed:14657280)
4. Gupta S et al. (2007) Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice. Cell, 129:957-68. (PubMed:17540175)
5. Lu W et al. (2002) Involvement of tissue plasminogen activator in onset and effector phases of experimental allergic encephalomyelitis. J Neurosci, 22:10781-9. (PubMed:12486171)
6. Papin J et al. (2004) Bioinformatics and cellular signaling. Curr Opin Biotechnol, 15:78-81. (PubMed:15102471)
7. Pons S et al. (1995) The structure and function of p55PIK reveal a new regulatory subunit for phosphatidylinositol 3-kinase. Mol Cell Biol, 15:4453-65. (PubMed:7542745)
8. Popkie AP et al. (2010) Phosphatidylinositol 3-kinase (PI3K) signaling via glycogen synthase kinase-3 (Gsk-3) regulates DNA methylation of imprinted loci. J Biol Chem, 285:41337-47. (PubMed:21047779)
9. Schubert M et al. (2003) Insulin receptor substrate-2 deficiency impairs brain growth and promotes tau phosphorylation. J Neurosci, 23:7084-92. (PubMed:12904469)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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