Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pknox1. (This text reflects annotations as of Wednesday, January 23, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of June 10, 2008. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pknox1
  • participates in the following biological processes:
    • positive regulation of transcription from RNA polymerase II promoter ^[4]
    • regulation of transcription from RNA polymerase II promoter ^[5]
  • performs the following molecular functions:
    • DNA binding ^{[1, 4, 6]}
    • RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity ^[5]
    • sequence-specific DNA binding ^[9]
  • is located in the following cellular components:
    • cytoplasm ^[8]
    • nucleus ^{[3, 8, 10]}
    • transcription factor complex ^{[1, 4, 5, 6, 10]}
• The gene product of Pknox1 has been shown to bind to the gene products of Meis1, Pbx1, Pbx2, Pbx3, Smad2, Smad3, Smad4. ^{[1, 4, 5]} Researchers have inferred, based on physical interactions, that the gene product of Pknox1
  • performs the following molecular functions:
    • protein heterodimerization activity ^[6]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pknox1
  • participates in the following biological processes:
    • angiogenesis ^[3]
    • camera-type eye development ^[3]
    • erythrocyte differentiation ^[3]
    • hemopoiesis ^[2]
    • T cell differentiation ^[7]
• Researchers have inferred, based on genetic interactions, that the gene product of Pknox1
  • performs the following molecular functions:
    • DNA binding based on interactions with Pbx1 ^[10]

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Summary text based on GO annotations supported by structural data

• Pknox1 encodes a protein or proteins that contain the following InterPro domains: Homeobox KN domain, Homeodomain, Homeodomain-like, indicating that the gene product:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • sequence-specific DNA binding transcription factor activity

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References

1. Bailey JS et al. (2004) Activin regulation of the follicle-stimulating hormone beta-subunit gene involves Smads and the TALE homeodomain proteins Pbx1 and Prep1. Mol Endocrinol, 18:1158-70. (PubMed:14764653)
2. Di Rosa P et al. (2007) The homeodomain transcription factor Prep1 (pKnox1) is required for hematopoietic stem and progenitor cell activity. Dev Biol, 311:324-34. (PubMed:17904118)
3. Ferretti E et al. (2006) Hypomorphic mutation of the TALE gene Prep1 (pKnox1) causes a major reduction of Pbx and Meis proteins and a pleiotropic embryonic phenotype. Mol Cell Biol, 26:5650-62. (PubMed:16847320)
4. Ferretti E et al. (1999) The PBX-regulating protein PREP1 is present in different PBX-complexed forms in mouse. Mech Dev, 83:53-64. (PubMed:10381567)
5. Jacobs Y et al. (1999) Trimeric association of Hox and TALE homeodomain proteins mediates Hoxb2 hindbrain enhancer activity. Mol Cell Biol, 19:5134-42. (PubMed:10373562)
6. Knoepfler PS et al. (1997) Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1. Proc Natl Acad Sci U S A, 94:14553-8. (PubMed:9405651)
7. Penkov D et al. (2005) Involvement of Prep1 in the alphabeta T-cell receptor T-lymphocytic potential of hematopoietic precursors. Mol Cell Biol, 25:10768-81. (PubMed:16314502)
8. Villaescusa JC et al. (2004) Expression of Hox cofactor genes during mouse ovarian follicular development and oocyte maturation. Gene, 330:1-7. (PubMed:15087118)
9. Yoshioka H et al. (2007) In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis. Mol Cell Biol, 27:7871-85. (PubMed:17875925)
10. Zhang X et al. (2006) Pax6 is regulated by Meis and Pbx homeoproteins during pancreatic development. Dev Biol, 300:748-57. (PubMed:17049510)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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