Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pla2g4a. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pla2g4a
  • participates in the following biological processes:
    • arachidonic acid secretion ^[1]
    • cellular response to antibiotic ^[2]
    • metabolic process ^[1]
  • performs the following molecular functions:
    • phospholipase A2 activity ^[1]
  • is located in the following cellular components:
    • cytosol ^{[1, 2]}
    • endoplasmic reticulum ^[2]
    • Golgi apparatus ^[2]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pla2g4a
  • participates in the following biological processes:
    • arachidonic acid secretion ^[2]
    • icosanoid biosynthetic process ^[2]
• Researchers have inferred, based on genetic interactions, that the gene product of Pla2g4a
  • participates in the following biological processes:
    • regulation of cell proliferation based on interactions with Apc ^[3]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pla2g4a:
  • participates in the following biological processes:
    • arachidonic acid metabolic process
    • decidualization
    • ovulation from ovarian follicle
    • positive regulation of apoptotic process
    • positive regulation of cell proliferation
    • positive regulation of inflammatory response
    • positive regulation of prostaglandin biosynthetic process
    • positive regulation of vesicle fusion
    • response to calcium ion
    • response to organic substance
    • surfactant homeostasis
  • performs the following molecular functions:
    • calcium ion binding
    • calcium-dependent phospholipase A2 activity
    • calcium-dependent phospholipid binding
    • phospholipase A2 activity
  • is located in the following cellular components:
    • cytoplasm
    • cytosol
    • intracellular membrane-bounded organelle
    • nucleus
    • perinuclear region of cytoplasm
    • zymogen granule

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Summary text based on GO annotations supported by structural data

• Pla2g4a encodes a protein or proteins that contain the following InterPro domains: Acyl transferase/acyl hydrolase/lysophospholipase, C2 calcium-dependent membrane targeting, C2 calcium/lipid-binding domain, CaLB, C2 membrane targeting protein, Lysophospholipase, catalytic domain, indicating that the gene product:
  • participates in the following biological processes:
    • phospholipid catabolic process
  • performs the following molecular functions:
    • phospholipase activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pla2g4a indicates that it:
  • participates in the following biological processes:
    • lipid catabolic process
    • lipid metabolic process
  • performs the following molecular functions:
    • hydrolase activity
    • metal ion binding
  • is located in the following cellular components:
    • cytoplasmic vesicle
• Pla2g4a has been associated with the Enzyme Commission numbers: 3.1.1.4, 3.1.1.5, which implies that the gene product:
  • performs the following molecular functions:
    • lysophospholipase activity

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References

1. Fonteh AN et al. (2000) Secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2 in murine bone marrow-derived mast cells. J Immunol, 165:2773-82. (PubMed:10946309)
2. Ghosh M et al. (2007) Function, activity, and membrane targeting of cytosolic phospholipase A(2)zeta in mouse lung fibroblasts. J Biol Chem, 282:11676-86. (PubMed:17293613)
3. Takaku K et al. (2000) Suppression of intestinal polyposis in ApcDelta 716 knockout mice by an additional mutation in the cytosolic phospholipase A2 gene J Biol Chem, 275:34013-6. (PubMed:10969066)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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