Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Epha8. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein encoded by this gene functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Epha8
  • participates in the following biological processes:
    • ephrin receptor signaling pathway ^{[3, 4, 6]}
    • neuron projection development ^[2]
    • neuron remodeling ^[6]
    • positive regulation of MAPK cascade ^[2]
    • positive regulation of phosphatidylinositol 3-kinase activity ^[3]
    • protein autophosphorylation ^{[1, 3]}
    • regulation of cell adhesion ^[1]
    • regulation of cell adhesion mediated by integrin ^[3]
    • substrate-dependent cell migration ^[6]
  • performs the following molecular functions:
    • GPI-linked ephrin receptor activity ^{[3, 4]}
  • is located in the following cellular components:
    • integral to plasma membrane ^[4]
    • neuron projection ^[6]
• The gene product of Epha8 has been shown to bind to the gene products of Efna2, Efna3, Efna5. ^{[1, 3, 4, 6]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Epha8
  • participates in the following biological processes:
    • axon guidance ^[5]

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Summary text based on GO annotations supported by structural data

• Epha8 encodes a protein or proteins that contain the following InterPro domains: Ephrin receptor ligand binding domain, Ephrin type-A receptor 8, Fibronectin, type III, Galactose-binding domain-like, Immunoglobulin-like fold, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine-threonine/tyrosine-protein kinase catalytic domain, Sterile alpha motif domain, Sterile alpha motif, type 1, Sterile alpha motif/pointed domain, Tyrosine-protein kinase, active site, Tyrosine-protein kinase, catalytic domain, Tyrosine-protein kinase, ephrin receptor, Tyrosine-protein kinase, receptor class V, conserved site, indicating that the gene product:
  • participates in the following biological processes:
    • ephrin receptor signaling pathway
    • protein phosphorylation
    • transmembrane receptor protein tyrosine kinase signaling pathway
  • performs the following molecular functions:
    • ATP binding
    • ephrin receptor activity
    • protein kinase activity
    • protein tyrosine kinase activity
    • transferase activity, transferring phosphorus-containing groups
  • is located in the following cellular components:
    • integral to membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Epha8 indicates that it:
  • participates in the following biological processes:
    • cell adhesion
    • multicellular organismal development
    • nervous system development
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • nucleotide binding
    • protein tyrosine kinase activity
    • transferase activity
  • is located in the following cellular components:
    • cell projection
    • endosome
    • integral to membrane
    • membrane
    • plasma membrane
• Epha8 has been associated with the Enzyme Commission numbers: 2.7.10.1, which implies that the gene product:
  • performs the following molecular functions:
    • transmembrane receptor protein tyrosine kinase activity

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References

1. Choi S et al. (1999) Phosphorylation at Tyr-838 in the kinase domain of EphA8 modulates Fyn binding to the Tyr-615 site by enhancing tyrosine kinase activity. Oncogene, 18:5413-22. (PubMed:10498895)
2. Gu C et al. (2005) The EphA8 receptor induces sustained MAP kinase activation to promote neurite outgrowth in neuronal cells. Oncogene, 24:4243-56. (PubMed:15782114)
3. Gu C et al. (2001) The EphA8 receptor regulates integrin activity through p110gamma phosphatidylinositol-3 kinase in a tyrosine kinase activity-independent manner. Mol Cell Biol, 21:4579-97. (PubMed:11416136)
4. Park S et al. (1997) The Eek receptor, a member of the Eph family of tyrosine protein kinases, can be activated by three different Eph family ligands. Oncogene, 14:533-42. (PubMed:9053851)
5. Park S et al. (1997) Aberrant axonal projections in mice lacking EphA8 (Eek) tyrosine protein kinase receptors. EMBO J, 16:3106-14. (PubMed:9214628)
6. Shin J et al. (2007) Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function. Mol Cell Biol, 27:8113-26. (PubMed:17875921)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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