Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Klc1. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Klc1
  • is located in the following cellular components:
    • axon ^[2]
    • ciliary rootlet ^[10]
    • cytoplasm ^[10]
    • cytoplasmic vesicle ^[2]
    • cytosol ^[7]
    • kinesin complex ^[7]
    • membrane-bounded organelle ^[6]
    • neuron projection ^[5]
• The gene product of Klc1 has been shown to bind to the gene products of Crocc, Kif5b, Mapk8ip2, Mapk8ip3, Syne4. ^{[1, 3, 7, 8, 9, 10]} Researchers have inferred, based on physical interactions, that the gene product of Klc1
  • performs the following molecular functions:
  • is located in the following cellular components:
    • ciliary rootlet ^[10]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Klc1
  • participates in the following biological processes:
    • axon cargo transport ^{[2, 5]}
    • stress granule disassembly ^[4]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Klc1:
  • participates in the following biological processes:
    • intracellular protein transport
    • protein localization to synapse
  • performs the following molecular functions:
    • tubulin binding
  • is located in the following cellular components:
    • axon
    • kinesin complex
    • membrane
    • neuron projection
    • neuronal cell body
    • vesicle

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Summary text based on GO annotations supported by structural data

• Klc1 encodes a protein or proteins that contain the following InterPro domains: Kinesin light chain, Kinesin light chain repeat, Rabaptin, GTPase-Rab5 binding domain, Tetratricopeptide repeat, Tetratricopeptide repeat-containing domain, Tetratricopeptide TPR-1, Tetratricopeptide TPR2, Tetratricopeptide-like helical, indicating that the gene product:
  • performs the following molecular functions:
    • microtubule motor activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Klc1 indicates that it:
  • is located in the following cellular components:
    • cytoskeleton
    • microtubule

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References

1. Bowman AB et al. (2000) Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Cell, 103:583-94. (PubMed:11106729)
2. Kamal A et al. (2001) Kinesin-mediated axonal transport of a membrane compartment containing beta-secretase and presenilin-1 requires APP. Nature, 414:643-8. (PubMed:11740561)
3. Kelkar N et al. (2000) Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Mol Cell Biol, 20:1030-43. (PubMed:10629060)
4. Loschi M et al. (2009) Dynein and kinesin regulate stress-granule and P-body dynamics. J Cell Sci, 122:3973-82. (PubMed:19825938)
5. Muresan Z et al. (2005) Coordinated transport of phosphorylated amyloid-beta precursor protein and c-Jun NH2-terminal kinase-interacting protein-1. J Cell Biol, 171:615-25. (PubMed:16301330)
6. Pigino G et al. (2003) Alzheimer's presenilin 1 mutations impair kinesin-based axonal transport. J Neurosci, 23:4499-508. (PubMed:12805290)
7. Rahman A et al. (1998) Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins. (Erratum pg. 24280) J Biol Chem, 273:15395-403. (PubMed:9624122)
8. Roux KJ et al. (2009) Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proc Natl Acad Sci U S A, 106:2194-9. (PubMed:19164528)
9. Verhey KJ et al. (2001) Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. J Cell Biol, 152:959-70. (PubMed:11238452)
10. Yang J et al. (2005) The ciliary rootlet interacts with kinesin light chains and may provide a scaffold for kinesin-1 vesicular cargos. Exp Cell Res, 309:379-89. (PubMed:16018997)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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