Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Ppp3ca. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Ppp3ca
  • participates in the following biological processes:
    • calcineurin-NFAT signaling cascade ^{[7, 8]}
    • dephosphorylation ^{[10, 13]}
    • protein import into nucleus ^[3]
    • response to stress ^[3]
    • transition between fast and slow fiber ^[8]
  • performs the following molecular functions:
    • phosphoprotein phosphatase activity ^[10]
  • is located in the following cellular components:
    • calcineurin complex ^[5]
    • cytosol ^[5]
    • mitochondrion ^[5]
    • nucleus ^[5]
    • Z disc ^[6]
• The gene product of Ppp3ca has been shown to bind to the gene products of Bcl2, Csrp3, Gabrg2, Itpr1, Ksr2, Mapt. ^{[4, 5, 6, 8, 9, 13, 14]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Ppp3ca
  • participates in the following biological processes:
    • calcium ion transport ^[1]
    • G1/S transition of mitotic cell cycle ^[1]
    • protein dephosphorylation ^[4]
    • regulation of synaptic transmission ^[12]
    • skeletal muscle fiber development ^[10]
• Researchers have inferred, based on genetic interactions, that the gene product of Ppp3ca
  • participates in the following biological processes:
    • calcium-mediated signaling based on interactions with Ksr2 ^[4]
    • positive regulation of transcription from RNA polymerase II promoter based on interactions with Nfatc3 ^[2]
    • regulation of excitatory postsynaptic membrane potential based on interactions with Grik2 ^[11]
  • is located in the following cellular components:
    • intracellular based on interactions with Ksr2 ^[4]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Ppp3ca:
  • participates in the following biological processes:
    • cellular response to drug
    • cellular response to glucose stimulus
    • negative regulation of insulin secretion
    • protein dephosphorylation
    • response to calcium ion
  • performs the following molecular functions:
    • calcium-dependent protein serine/threonine phosphatase activity
    • calmodulin binding
    • calmodulin-dependent protein phosphatase activity
    • drug binding
    • enzyme binding
    • phosphoprotein phosphatase activity
    • protein dimerization activity
    • protein heterodimerization activity
    • protein serine/threonine phosphatase activity
  • is located in the following cellular components:
    • calcineurin complex
    • cytoplasm
    • cytosol
    • membrane
    • nucleus

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Summary text based on GO annotations supported by structural data

• Ppp3ca encodes a protein or proteins that contain the following InterPro domains: Metallophosphoesterase domain, Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase, indicating that the gene product:
  • performs the following molecular functions:
    • hydrolase activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Ppp3ca indicates that it:
  • performs the following molecular functions:
    • hydrolase activity
    • metal ion binding

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References

1. Afroze T et al. (2003) Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am J Physiol Cell Physiol, 285:C88-95. (PubMed:12660151)
2. Alfieri CM et al. (2007) Developmental regulation of the mouse IGF-I exon 1 promoter region by calcineurin activation of NFAT in skeletal muscle. Am J Physiol Cell Physiol, 292:C1887-94. (PubMed:17229811)
3. Antos CL et al. (2002) Activated glycogen synthase-3 beta suppresses cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A, 99:907-12. (PubMed:11782539)
4. Dougherty MK et al. (2009) KSR2 is a calcineurin substrate that promotes ERK cascade activation in response to calcium signals. Mol Cell, 34:652-62. (PubMed:19560418)
5. Erin N et al. (2003) Calcium-dependent interaction of calcineurin with Bcl-2 in neuronal tissue. Neuroscience, 117:541-55. (PubMed:12617961)
6. Heineke J et al. (2005) Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc. Proc Natl Acad Sci U S A, 102:1655-60. (PubMed:15665106)
7. Ikeda S et al. (2009) MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes. Mol Cell Biol, 29:2193-204. (PubMed:19188439)
8. Kielbasa OM et al. (2011) Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration. FASEB J, 25:2276-86. (PubMed:21427212)
9. Lin SS et al. (2006) PP2A regulates BCL-2 phosphorylation and proteasome-mediated degradation at the endoplasmic reticulum. J Biol Chem, 281:23003-12. (PubMed:16717086)
10. Oh M et al. (2005) Calcineurin is necessary for the maintenance but not embryonic development of slow muscle fibers. Mol Cell Biol, 25:6629-38. (PubMed:16024798)
11. Rebola N et al. (2007) Short-term plasticity of kainate receptor-mediated EPSCs induced by NMDA receptors at hippocampal mossy fiber synapses. J Neurosci, 27:3987-93. (PubMed:17428973)
12. Townsend M et al. (2004) Retina-driven dephosphorylation of the NR2A subunit correlates with faster NMDA receptor kinetics at developing retinocollicular synapses. J Neurosci, 24:11098-107. (PubMed:15590926)
13. Wang J et al. (2003) Interaction of calcineurin and type-A GABA receptor gamma 2 subunits produces long-term depression at CA1 inhibitory synapses. J Neurosci, 23:826-36. (PubMed:12574411)
14. Yu DY et al. (2008) Tau binds both subunits of calcineurin, and binding is impaired by calmodulin. Biochim Biophys Acta, 1783:2255-61. (PubMed:18639592)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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