Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Was. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Was
  • participates in the following biological processes:
    • actin filament polymerization ^[1]
    • actin filament-based movement ^[1]
    • actin polymerization or depolymerization ^[1]
    • endosomal transport ^[1]
  • performs the following molecular functions:
    • identical protein binding ^[1]
  • is located in the following cellular components:
    • vesicle membrane ^[1]
• The gene product of Was has been shown to bind to the gene products of Dlg1, Ipo5, Lck, Rhoj, Src, Wipf1, Zap70. ^{[3, 4, 5, 6]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Was
  • participates in the following biological processes:
    • T cell activation ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Was:
  • participates in the following biological processes:
    • immune response
  • performs the following molecular functions:
    • identical protein binding

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Summary text based on GO annotations supported by structural data

• Was encodes a protein or proteins that contain the following InterPro domains: EVH1, PAK-box/P21-Rho-binding, Pleckstrin homology-like domain, WH2 domain, Wiscott-Aldrich syndrome, C-terminal, indicating that the gene product:
  • participates in the following biological processes:
    • protein complex assembly
  • performs the following molecular functions:
    • actin binding
    • small GTPase regulator activity
  • is located in the following cellular components:
    • actin cytoskeleton

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Was indicates that it:
  • is located in the following cellular components:
    • cytoplasm
    • cytoskeleton

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References

1. Benesch S et al. (2002) Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2. J Biol Chem, 277:37771-6. (PubMed:12147689)
2. Krawczyk C et al. (2000) Cbl-b is a negative regulator of receptor clustering and raft aggregation in T cells. Immunity, 13:463-73. (PubMed:11070165)
3. Ma T et al. (2008) Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide containing pTyr294 amino acid. J Mol Signal, 3:4. (PubMed:18289379)
4. Papin J et al. (2004) Bioinformatics and cellular signaling. Curr Opin Biotechnol, 15:78-81. (PubMed:15102471)
5. Round JL et al. (2005) Dlgh1 coordinates actin polymerization, synaptic T cell receptor and lipid raft aggregation, and effector function in T cells. J Exp Med, 201:419-30. (PubMed:15699074)
6. Vignal E et al. (2000) Characterization of TCL, a new GTPase of the rho family related to TC10 andCcdc42. J Biol Chem, 275:36457-64. (PubMed:10967094)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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