Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Col4a3. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Col4a3
  • participates in the following biological processes:
    • cell surface receptor signaling pathway ^[2]
    • negative regulation of angiogenesis ^[5]
  • is located in the following cellular components:
    • basement membrane ^{[3, 4, 6]}
    • collagen type IV ^[7]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Col4a3
  • participates in the following biological processes:
    • glomerular basement membrane development ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Col4a3:
  • participates in the following biological processes:
    • activation of cysteine-type endopeptidase activity involved in apoptotic process
    • cell proliferation
    • induction of apoptosis
    • negative regulation of angiogenesis
  • performs the following molecular functions:
    • integrin binding
  • is located in the following cellular components:
    • basement membrane
    • collagen type IV

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Summary text based on GO annotations supported by structural data

• Col4a3 encodes a protein or proteins that contain the following InterPro domains: C-type lectin fold, Collagen IV, non-collagenous, Collagen triple helix repeat, indicating that the gene product:
  • performs the following molecular functions:
    • extracellular matrix structural constituent
  • is located in the following cellular components:
    • collagen

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Col4a3 indicates that it:
  • participates in the following biological processes:
    • cell adhesion
  • is located in the following cellular components:
    • collagen
    • extracellular region
    • proteinaceous extracellular matrix

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References

1. Abrahamson DR et al. (2003) Laminin-1 reexpression in Alport mouse glomerular basement membranes. Kidney Int, 63:826-34. (PubMed:12631063)
2. Hamano Y et al. (2003) Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaV beta3 integrin. Cancer Cell, 3:589-601. (PubMed:12842087)
3. Manabe R et al. (2008) Transcriptome-based systematic identification of extracellular matrix proteins. Proc Natl Acad Sci U S A, 105:12849-54. (PubMed:18757743)
4. Meyer zum Gottesberge AM et al. (2005) Abnormal basement membrane in the inner ear and the kidney of the Mpv17-/- mouse strain: ultrastructural and immunohistochemical investigations. Histochem Cell Biol, 124:507-16. (PubMed:16041630)
5. Miyoshi T et al. (2006) Tumor-specific expression of the RGD-alpha3(IV)NC1 domain suppresses endothelial tube formation and tumor growth in mice. FASEB J, 20:1904-6. (PubMed:16877525)
6. Nagai N et al. (2001) Localization of type IV collagen a 1 to a 6 chains in basement membrane during mouse molar germ development. Int J Dev Biol, 45:827-31. (PubMed:11732842)
7. Shindo T et al. (2002) Kruppel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling. Nat Med, 8:856-63. (PubMed:12101409)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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