Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Limk1. (This text reflects annotations as of Thursday, January 16, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Limk1
  • is located in the following cellular components:
    • cytoplasm ^{[1, 5]}
    • focal adhesion ^{[1, 3]}
    • neuron projection ^[5]
    • nucleus ^{[1, 3]}
• The gene product of Limk1 has been shown to bind to the gene products of Pxn, Rlim. ^{[1, 2, 3, 5]} Researchers have inferred, based on physical interactions, that the gene product of Limk1
  • performs the following molecular functions:
    • protein heterodimerization activity ^[1]
• Researchers have inferred, based on genetic interactions, that the gene product of Limk1
  • participates in the following biological processes:
    • positive regulation of axon extension based on interactions with Rnf6 ^[5]
    • positive regulation of stress fiber assembly based on interactions with Braf ^[4]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Limk1:
  • participates in the following biological processes:
    • negative regulation of ubiquitin-protein ligase activity
    • positive regulation of actin filament bundle assembly
    • protein phosphorylation
  • performs the following molecular functions:
    • heat shock protein binding
  • is located in the following cellular components:
    • axonal growth cone
    • cytoplasm
    • Golgi apparatus
    • nucleus
    • perinuclear region of cytoplasm

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Summary text based on GO annotations supported by structural data

• Limk1 encodes a protein or proteins that contain the following InterPro domains: PDZ domain, Protein kinase, ATP binding site, Protein kinase, catalytic domain, Protein kinase-like domain, Serine-threonine/tyrosine-protein kinase catalytic domain, Zinc finger, LIM-type, indicating that the gene product:
  • performs the following molecular functions:
    • ATP binding
    • protein kinase activity
    • transferase activity, transferring phosphorus-containing groups
    • zinc ion binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Limk1 indicates that it:
  • participates in the following biological processes:
    • phosphorylation
  • performs the following molecular functions:
    • ATP binding
    • kinase activity
    • metal ion binding
    • nucleotide binding
    • protein serine/threonine kinase activity
    • transferase activity

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References

1. Acevedo K et al. (2006) LIM kinase 2 is widely expressed in all tissues. J Histochem Cytochem, 54:487-501. (PubMed:16399995)
2. Bach I et al. (1999) RLIM inhibits functional activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. Nat Genet, 22:394-9. (PubMed:10431247)
3. Foletta VC et al. (2004) LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues. Exp Cell Res, 294:392-405. (PubMed:15023529)
4. Pritchard CA et al. (2004) B-Raf acts via the ROCKII/LIMK/cofilin pathway to maintain actin stress fibers in fibroblasts. Mol Cell Biol, 24:5937-52. (PubMed:15199148)
5. Tursun B et al. (2005) The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones. Genes Dev, 19:2307-19. (PubMed:16204183)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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