Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Lmo2. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Lmo2
  • performs the following molecular functions:
    • chromatin binding ^{[3, 6]}
    • DNA binding ^[7]
  • is located in the following cellular components:
    • nucleus ^{[6, 7]}
• The gene product of Lmo2 has been shown to bind to the gene products of Elf2, Gata1, Ldb1, Rlim, Tal1. ^{[1, 2, 5, 7, 8]} Researchers have inferred, based on physical interactions, that the gene product of Lmo2
  • performs the following molecular functions:
  • is located in the following cellular components:
    • protein complex ^[7]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Lmo2
  • participates in the following biological processes:
    • negative regulation of erythrocyte differentiation ^[7]
• Researchers have inferred, based on genetic interactions, that the gene product of Lmo2
  • participates in the following biological processes:
    • embryonic hemopoiesis based on interactions with Gata3 ^[4]
    • positive regulation of transcription from RNA polymerase II promoter based on interactions with Gata1, Gata2, Ldb1, Tal1, Tcf3 ^{[3, 6]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Lmo2:
  • participates in the following biological processes:
    • cellular response to thyroid hormone stimulus
    • mRNA transcription from RNA polymerase II promoter
    • positive regulation of transcription from RNA polymerase II promoter
  • performs the following molecular functions:
    • bHLH transcription factor binding
    • cofactor binding
    • E-box binding
    • RNA polymerase II activating transcription factor binding
    • RNA polymerase II regulatory region sequence-specific DNA binding
    • RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription
    • RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
    • sequence-specific DNA binding RNA polymerase II transcription factor activity
  • is located in the following cellular components:
    • transcription factor complex

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Summary text based on GO annotations supported by structural data

• Lmo2 encodes a protein or proteins that contain the following InterPro domains: Zinc finger, LIM-type, indicating that the gene product:
  • performs the following molecular functions:
    • zinc ion binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Lmo2 indicates that it:
  • performs the following molecular functions:
    • metal ion binding

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References

1. Bach I et al. (1999) RLIM inhibits functional activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. Nat Genet, 22:394-9. (PubMed:10431247)
2. Joshi K et al. (2009) LMO4 controls the balance between excitatory and inhibitory spinal V2 interneurons. Neuron, 61:839-51. (PubMed:19323994)
3. Landry JR et al. (2008) Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver. Blood, 111:3005-14. (PubMed:18184866)
4. Manaia A et al. (2000) Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites. Development, 127:643-53. (PubMed:10631184)
5. Osada H et al. (1995) Association of erythroid transcription factors: complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1. Proc Natl Acad Sci U S A, 92:9585-9. (PubMed:7568177)
6. Tripic T et al. (2009) SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood, 113:2191-201. (PubMed:19011221)
7. Visvader JE et al. (1997) The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation. Proc Natl Acad Sci U S A, 94:13707-12. (PubMed:9391090)
8. Wilkinson DA et al. (1997) Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia. Leukemia, 11:86-96. (PubMed:9001422)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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