Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Runx3. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Runx3
  • participates in the following biological processes:
    • interferon-gamma production ^[5]
    • mRNA transcription from RNA polymerase II promoter ^[8]
    • negative regulation of transcription from RNA polymerase II promoter ^[8]
  • performs the following molecular functions:
    • DNA binding ^[7]
    • RNA polymerase II regulatory region sequence-specific DNA binding ^[8]
    • sequence-specific DNA binding RNA polymerase II transcription factor activity ^[8]
  • is located in the following cellular components:
    • nuclear chromatin ^[8]
    • nucleus ^[1]
• The gene product of Runx3 has been shown to bind to the gene products of Gata3. ^[5]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Runx3
  • participates in the following biological processes:
    • axon guidance ^[2]
    • hair follicle morphogenesis ^[4]
    • induction of apoptosis ^[3]
    • negative regulation of cell cycle ^[3]
    • negative regulation of epithelial cell proliferation ^[3]
    • neuron projection development ^[2]
• Researchers have inferred, based on genetic interactions, that the gene product of Runx3
  • participates in the following biological processes:
    • cell maturation based on interactions with Runx2 ^[6]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Runx3:
  • participates in the following biological processes:
    • protein phosphorylation
  • is located in the following cellular components:
    • cytoplasm
    • nucleus

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Summary text based on GO annotations supported by structural data

• Runx3 encodes a protein or proteins that contain the following InterPro domains: Acute myeloid leukemia 1 (AML 1)/Runt, Acute myeloid leukemia 1 protein (AML 1)/Runt, p53-like transcription factor, DNA-binding, p53/RUNT-type transcription factor, DNA-binding domain, Runx inhibition, Transcription factor, Runt-related, RUNX, indicating that the gene product:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • ATP binding
    • sequence-specific DNA binding transcription factor activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Runx3 indicates that it:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent

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References

1. Brenner O et al. (2004) Loss of Runx3 function in leukocytes is associated with spontaneously developed colitis and gastric mucosal hyperplasia. Proc Natl Acad Sci U S A, 101:16016-21. (PubMed:15514019)
2. Inoue K et al. (2002) Runx3 controls the axonal projection of proprioceptive dorsal root ganglion neurons. Nat Neurosci, 5:946-54. (PubMed:12352981)
3. Li QL et al. (2002) Causal Relationship between the Loss of RUNX3 Expression and Gastric Cancer. Cell, 109:113-24. (PubMed:11955451)
4. Raveh E et al. (2005) Runx3 is involved in hair shape determination. Dev Dyn, 233:1478-87. (PubMed:15937937)
5. Yagi R et al. (2010) The transcription factor GATA3 actively represses RUNX3 protein-regulated production of interferon-gamma. Immunity, 32:507-17. (PubMed:20399120)
6. Yoshida CA et al. (2004) Runx2 and Runx3 are essential for chondrocyte maturation, and Runx2 regulates limb growth through induction of Indian hedgehog. Genes Dev, 18:952-63. (PubMed:15107406)
7. Zhang L et al. (2003) Mutagenesis of the Runt domain defines two energetic hot spots for heterodimerization with the core binding factor beta subunit. J Biol Chem, 278:33097-104. (PubMed:12807883)
8. Zheng L et al. (2007) Runx3 negatively regulates Osterix expression in dental pulp cells. Biochem J, 405:69-75. (PubMed:17352693)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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