Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Pou3f4. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the POU-III class of neural transcription factors. This family member plays a role in inner ear development. The protein is thought to be involved in the mediation of epigenetic signals which induce striatal neuron-precursor differentiation. Mutations in this gene are associated with X chromosome-linked nonsyndromic mixed deafness. [provided by RefSeq, Dec 2012]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Pou3f4
  • performs the following molecular functions:
    • sequence-specific DNA binding ^[2]
  • is located in the following cellular components:
    • intracellular part ^[3]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Pou3f4
  • participates in the following biological processes:
    • forebrain neuron differentiation ^[6]
    • inner ear development ^{[4, 7]}
    • sensory perception of sound ^{[4, 5]}
• Researchers have inferred, based on genetic interactions, that the gene product of Pou3f4
  • participates in the following biological processes:
    • cochlea morphogenesis based on interactions with Tbx1 ^[1]
    • negative regulation of mesenchymal cell apoptotic process based on interactions with Tbx1 ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Pou3f4:
  • participates in the following biological processes:
    • transcription from RNA polymerase II promoter
  • performs the following molecular functions:
    • AT DNA binding
    • double-stranded DNA binding
    • sequence-specific DNA binding transcription factor activity
  • is located in the following cellular components:
    • nucleus

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Summary text based on GO annotations supported by structural data

• Pou3f4 encodes a protein or proteins that contain the following InterPro domains: Homeobox, conserved site, Homeodomain, Homeodomain-like, Lambda repressor-like, DNA-binding domain, POU domain, POU-specific, Transcription factor, homeobox/POU, indicating that the gene product:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • DNA binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Pou3f4 indicates that it:
  • participates in the following biological processes:
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent
  • performs the following molecular functions:
    • DNA binding

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References

1. Braunstein EM et al. (2008) Cooperative function of Tbx1 and Brn4 in the periotic mesenchyme is necessary for cochlea formation. J Assoc Res Otolaryngol, 9:33-43. (PubMed:18231833)
2. Castro DS et al. (2006) Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell, 11:831-44. (PubMed:17141158)
3. Jensen J et al. (2000) Independent development of pancreatic alpha- and beta-cells from neurogenin3-expressing precursors: a role for the notch pathway in repression of premature differentiation. Diabetes, 49:163-76. (PubMed:10868931)
4. Phippard D et al. (2000) The sex-linked fidget mutation abolishes Brn4/Pou3f4 gene expression in the embryonic inner ear. Hum Mol Genet, 9:79-85. (PubMed:10587581)
5. Samadi DS et al. (2005) Mutation of the POU-domain gene Brn4/Pou3f4 affects middle-ear sound conduction in the mouse. Hear Res, 199:11-21. (PubMed:15574296)
6. Shimazaki T et al. (1999) A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation. EMBO J, 18:444-56. (PubMed:9889200)
7. Sobol SE et al. (2005) Abnormal mesenchymal differentiation in the superior semicircular canal of brn4/pou3f4 knockout mice. Arch Otolaryngol Head Neck Surg, 131:41-5. (PubMed:15655183)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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